| Hirudin versus heparin for use in whole blood in vitro biocompatibility models. | |
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MedLine Citation:
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PMID: 18470919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heparin has traditionally been a widely used anticoagulant in blood research, but has been shown to be inappropriate for work with the complement system because of its complement-interacting properties. In this work, we have compared the effects of heparin with those of the specific thrombin inhibitor hirudin on complement and blood cells in vitro. Whole blood collected in the presence of hirudin (50 microg/mL) or heparin (1 IU/mL) was incubated in the slide chamber model. The plasma was analyzed for complement activation markers C3a and sC5b-9, and the polyvinylchloride test slides were stained for adhering cells. The integrity of the complement system was tested by incubating serum and hirudin-treated plasma in the presence of various activating agents. In contrast to heparin, the addition of hirudin generally preserved the complement reactivity, and complement activation in hirudin plasma closely resembled that in normal serum. Importantly, immunochemical staining of surface-bound cells demonstrated the inducible expression of tissue factor on bound monocytes from hirudin-treated blood, an effect that was completely abolished in heparin-treated blood. Our results indicate that hirudin as an anticoagulant produces more physiological conditions than heparin, making hirudin well-suited for in vitro studies, especially those addressing the regulation of cellular processes. |
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Authors:
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Fredrik Bexborn; Anna E Engberg; Kerstin Sandholm; Tom Eirik Mollnes; Jaan Hong; Kristina Nilsson Ekdahl |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 89 ISSN: 1552-4965 ISO Abbreviation: - Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-11 Completed Date: 2009-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 951-9 Citation Subset: IM |
Copyright Information:
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Copyright 2008 Wiley Periodicals, Inc. |
Affiliation:
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School of Pure and Applied Natural Sciences, University of Kalmar, Kalmar, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials
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pharmacology* Blood Platelets / drug effects Cell Adhesion / drug effects Complement Activation / drug effects Complement C3a / metabolism Complement Membrane Attack Complex / metabolism Heparin / pharmacology* Hirudins / blood, pharmacology* Humans Leukocytes / cytology, drug effects Models, Biological* Monocytes / cytology, drug effects Polyvinyl Chloride / pharmacology Serum Solubility / drug effects Thromboplastin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Complement Membrane Attack Complex; 0/Hirudins; 80295-42-7/Complement C3a; 9002-86-2/Polyvinyl Chloride; 9005-49-6/Heparin; 9035-58-9/Thromboplastin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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