| Hirudin C-terminal fragments inhibit thrombin induced neutrophil chemotaxis. | |
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MedLine Citation:
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PMID: 1641817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Since native hirudin blocks the thrombin induced chemotaxis response of neutrophils, we examined whether hirudin C-terminal peptides were also capable of this inhibition. The studies showed that thrombin induced human neutrophil chemotaxis was effectively blocked by the C-terminal hirudin peptide analogs, Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-Leu-Gln (12-mer[54-65]) and Thr-Pro-Lys-Pro-Gln-Ser-His-Asn-Asp-Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu- Tyr- Leu-Gln (21-mer[45-65]). Furthermore, neither peptide had an effect on formyl-L-methionyl-L-leucyl-L-phenylalanine induced chemotaxis. The results suggest that binding of the hirudin C-terminal peptides block the thrombin chemotactic domain. |
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Authors:
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J K Rowand; P Marucha; L J Berliner |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Thrombosis and haemostasis Volume: 67 ISSN: 0340-6245 ISO Abbreviation: Thromb. Haemost. Publication Date: 1992 Mar |
Date Detail:
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Created Date: 1992-08-28 Completed Date: 1992-08-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7608063 Medline TA: Thromb Haemost Country: GERMANY |
Other Details:
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Languages: eng Pagination: 289-91 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Ohio State University, Columbus 43210-1173. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Chemotaxis, Leukocyte / drug effects* Hirudins / chemistry, pharmacology* Humans Molecular Sequence Data N-Formylmethionine Leucyl-Phenylalanine / pharmacology Neutrophils / drug effects Peptide Fragments / pharmacology* Thrombin / antagonists & inhibitors* |
| Grant Support | |
ID/Acronym/Agency:
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HL24549/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hirudins; 0/Peptide Fragments; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; EC 3.4.21.5/Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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