Document Detail


Hippuric acid test using 13C-labelling and NMR spectroscopy.
MedLine Citation:
PMID:  11350017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This article describes a 13C-labelling and nuclear magnetic resonance approach for hippuric acid test which is potentially useful for evaluating liver reserve. In this approach, urine samples collected after ingestion of 13C-labelled benzoic acid were directly analysed by 13C nuclear magnetic resonance spectroscopy, and the excreted 13C-labelled hippuric acid formed from the administered benzoic acid was quantitated. The amount of labelled hippuric acid excreted in a specified time can be a useful index of liver reserve. In this study, the feasibility of the nuclear magnetic resonance approach has been investigated in several healthy subjects. This approach is simple and convenient compared with conventional analytical procedures, because no chromatographic separation is required. The approach could give new insights into the liver reserve, because the benzoic acid conversion to hippuric acid intimately relates to the hepatic energy metabolism. This measurement can be conducted at a wide range of dosages without interference from endogenous hippuric acid.
Authors:
K Akira; T Hashimoto
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Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Clinical chemistry and laboratory medicine : CCLM / FESCC     Volume:  39     ISSN:  1434-6621     ISO Abbreviation:  Clin. Chem. Lab. Med.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-05-14     Completed Date:  2001-10-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9806306     Medline TA:  Clin Chem Lab Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  215-7     Citation Subset:  IM    
Affiliation:
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan. akira@ps.toyaku.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Adult
Benzoic Acid / administration & dosage,  diagnostic use,  metabolism*
Carbon Isotopes / analysis*
Energy Metabolism / physiology
Hippurates / metabolism,  urine*
Humans
Liver / metabolism*
Liver Function Tests
Magnetic Resonance Spectroscopy
Male
Middle Aged
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Hippurates; 495-69-2/hippuric acid; 65-85-0/Benzoic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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