Document Detail

Hippocampal neurotrophic factors influence the perikaryal size of septal acetylcholinesterase-containing neurons in culture.
MedLine Citation:
PMID:  2253329     Owner:  NLM     Status:  MEDLINE    
The septal neurons were cultured under the following conditions: (1) treated with 7S nerve growth factor (NGF) (50 ng/ml); (2) grown with hippocampal cell-conditioned medium supernatant; (3) cocultivated with hippocampal cells; (4) cocultivated with cerebellar cells; (5) no treatment. Acetylcholinesterase histochemistry was used to identify cholinergic cells after pretreatment with diisopropylfluorophosphate. The mean values of the perikaryal major axis and minor axis at day 14 of culture were significantly larger in septal cells cocultivated with hippocampal cells than in septal cells grown under other conditions. NGF-treated septal cells showed a smaller, but significant, increase in the mean value of the major axis of these neurons.
K Harada; R Shingai; H Ito
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Brain research. Developmental brain research     Volume:  55     ISSN:  0165-3806     ISO Abbreviation:  Brain Res. Dev. Brain Res.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1991-01-24     Completed Date:  1991-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908639     Medline TA:  Brain Res Dev Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  293-7     Citation Subset:  IM    
Department of Neurosurgery, Yamaguchi University School of Medicine, Ube, Japan.
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MeSH Terms
Acetylcholinesterase / analysis*
Cells, Cultured
Hippocampus / embryology,  physiology*
Nerve Growth Factors / physiology*
Neurons / enzymology,  ultrastructure*
Septum Pellucidum / cytology,  enzymology,  ultrastructure*
Reg. No./Substance:
0/Nerve Growth Factors; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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