Document Detail

Hippocampal cell proliferation across the day: increase by running wheel activity, but no effect of sleep and wakefulness.
MedLine Citation:
PMID:  16214238     Owner:  NLM     Status:  MEDLINE    
The present study investigated whether proliferation of hippocampal progenitors is subject to circadian modulation. Mice were perfused using 3h intervals throughout the light-dark cycle and brains were stained for Ki-67. Since Ki-67 is not expressed during the G0 phase of the cell cycle, we expected a decline in Ki-67 expression at the moment cells synchronously exit the cell cycle. However, despite the fact that various hippocampal factors fluctuate across the day, the number of dividing cells remained constant. In a second experiment, we studied whether disturbance of normal sleep affected the stable rate in cell proliferation. Our data show that 12h of sleep deprivation during the light phase did not influence proliferating cell number. A third experiment investigated whether physical activity, a condition known to enhance hippocampal cell proliferation, caused an elevation of the steady baseline number of proliferating progenitors, or a peak directly following the active phase of the animals. Mice were housed with a running wheel for 9 days. On the last day, animals were sacrificed either directly before or directly after the active phase. Exercise significantly promoted cell proliferation and this effect appeared to be strongest directly after the active period and to disappear during the resting phase. Our data suggest that hippocampal cell proliferation is not synchronized under basal conditions and is unchanged by sleep deprivation. However, running affected cell proliferation differentially at two times of day. These data demonstrate that the steady rate in cell proliferation is not indispensable, but can be changed by behavioral activity.
Karin van der Borght; Francesca Ferrari; Karin Klauke; Viktor Roman; Robbert Havekes; Andrea Sgoifo; Eddy A van der Zee; Peter Meerlo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-07
Journal Detail:
Title:  Behavioural brain research     Volume:  167     ISSN:  0166-4328     ISO Abbreviation:  Behav. Brain Res.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-17     Completed Date:  2006-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004872     Medline TA:  Behav Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  36-41     Citation Subset:  IM    
Department of Molecular Neurobiology, Graduate School of Behavioral and Cognitive Neurosciences, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands.
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MeSH Terms
Analysis of Variance
Behavior, Animal / physiology
Bromodeoxyuridine / metabolism
Cell Count / methods
Cell Proliferation*
Hippocampus / cytology*
Immunohistochemistry / methods
Ki-67 Antigen / metabolism
Mice, Inbred C57BL
Neurons / physiology*
Physical Conditioning, Animal
Running / physiology*
Sleep / physiology*
Sleep Deprivation
Time Factors
Wakefulness / physiology*
Reg. No./Substance:
0/Ki-67 Antigen; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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