Document Detail


Highly sensitive and rapid radioimmunoassay for aldosterone in plasma and cell culture medium.
MedLine Citation:
PMID:  2776302     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A radioimmunoassay for aldosterone was developed using a sensitive and specific antibody and 125Iodinated-aldosterone. This assay could be used for direct determination of aldosterone in cell culture medium or after extraction of aldosterone from plasma by solid phase procedure using C18 Sep-pak cartridges. The very low cross-reactivity of the antibody with cortisol and corticosterone (0.005% and 0.04% respectively) would allow the direct determination of aldosterone in cell culture medium without any prior extraction step. Since the incubation is performed at room temperature for 1 h and then, at 4 degrees C for 15 min, the results can be obtained in less than 3 h. The assay was linear from 0.5 fmol/tube to 1500 fmol/tube with an ED50 at 30 fmol/tube. The accuracy of the assay estimated using spiked plasma samples with a known amount of aldosterone give a coefficient of correlation of 0.97 (n = 10) between the aldosterone concentrations found and expected levels. The within-assay variability for plasma aldosterone varied from 4.7 to 11.1% and the between-assay variability ranged from 13.9 to 14.2%. The coefficient of correlation between plasma aldosterone measured by this new assay or by a current assay was 0.8 (n = 43). In summary, the combination of a shortened incubation time with a simple solid phase extraction for aldosterone in serum samples represents the major advantage of the present assay over the current methodology which usually requires a chromatographic separation of the mineralocorticoid prior to radioimmunoassay. Therefore this assay would be useful in experimental studies as well as in clinical laboratory.
Authors:
M Brochu; J Féthière; M Roy; H Ong; A De Léan
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical biochemistry     Volume:  22     ISSN:  0009-9120     ISO Abbreviation:  Clin. Biochem.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-10-23     Completed Date:  1989-10-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  289-92     Citation Subset:  IM    
Affiliation:
Molecular Pharmacology Laboratory, Clinical Research Institute of Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Aldosterone / blood*
Antibody Specificity
Cross Reactions
Culture Media / analysis
Humans
Immunoassay / methods
Radioimmunoassay*
Chemical
Reg. No./Substance:
0/Culture Media; 52-39-1/Aldosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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