| Highly sensitive measurement of lipid molecular species from biological samples by fluorimetric detection coupled to high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 9653944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As the molecular species composition of glycerophospholipids provides more valuable information than the corresponding fatty acid composition, we have applied a fluorimetric detection (360 and 460 nm for excitation and emission wavelengths, respectively) of anthroyl derivatives of diradylglycerol species to minor phospholipid classes and subclasses from biological samples. Diacylglycerol species were obtained by phospholipase C treatment of phosphatidylcholine subclasses and phosphatidic acid extracted from rat thymocytes. Subpicomole measurements of molecular species from the minor subclass alkenylacylglycerophosphocholine could be achieved (e.g. 0.4 pmol of the 18:1/20:5 species). Such a sensitivity allowed study of the molecular species composition of another minor phospholipid, phosphatidic acid, and to evaluation of its alteration in mitogen-stimulated thymocytes as compared to unstimulated ones. Finally, we report that such a measurement is also applicable to other minor bioactive lipids with a hydroxyl group available, namely hydroxyeicosatetraenoates (HETEs), with a similar gain of sensitivity over conventional UV detection. Overall, these measurements, especially those of phospholipid molecular species, are sensitive, reliable and meaningful for precursor-product relationship between phospholipids. |
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Authors:
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C Thévenon; S El Bawab; B Chantegrel; M Lagarde |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 708 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1998-08-24 Completed Date: 1998-08-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 39-47 Citation Subset: IM |
Affiliation:
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INSERM U352, Biochimie and Pharmacologie, INSA-Lyon, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid / methods* Fatty Acids / analysis Fluorometry* Phosphatidic Acids / analysis Phosphatidylcholines / analysis Phospholipids / analysis* Rats Sensitivity and Specificity Thymus Gland / chemistry, cytology |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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