| Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip. | |
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MedLine Citation:
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PMID: 21431240 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We demonstrate a compact, low cost and practical fluorescence detection system for lab-on-a-chip applications. The system comprises a commercially available InGaN light emitting diode (501 nm) as light source, an organic or silicon photodiode detector, absorptive dye coated colour filters and linear and reflective polarisers. An injection moulded polystyrene microfluidic chip is used as the platform for fluorescence immunoassays for cardiac markers myoglobin and CK-MB. The optical limit of detection (LOD) is measured using a TransFluoSphere® suspension at 5.6 × 10(4) beads µl(-1) which can be equated to ∼3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml(-1) for both myoglobin and CK-MB. |
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Authors:
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Gihan Ryu; Jingsong Huang; Oliver Hofmann; Claire A Walshe; Jasmine Y Y Sze; Gareth D McClean; Alan Mosley; Simon J Rattle; John C Demello; Andrew J Demello; Donal D C Bradley |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-24 |
Journal Detail:
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Title: Lab on a chip Volume: - ISSN: 1473-0189 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Molecular Vision Ltd. BioIncubator Unit, Bessemer Building, Imperial College London, London, SW7 2BP, UK. simon.rattle@molecularvision.co.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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