Document Detail

Highly sensitive PCR-based detection method specific for Aspergillus flavus in wheat flour.
MedLine Citation:
PMID:  18484303     Owner:  NLM     Status:  MEDLINE    
Aspergillus flavus is frequently found in food, producing a wide variety of toxins, aflatoxins being the most relevant in food safety. A specific PCR-based protocol for this species is described which allowed discrimination from other closely related species having different profiles of secondary metabolites from the Aspergillus Section Flavi, particularly A. parasiticus. The specific primers were designed on the multi-copy internal transcribed region of the rDNA unit (ITS1-5.8S-ITS2 rDNA) and were tested in a wide sample of related species and other fungal species commonly found in food. The PCR assay was coupled with a fungal enrichment and a DNA extraction method for wheat flour to enhance the sensitivity of the diagnostic protocol. The results indicated that the critical PCR amplification product was clearly observed for wheat flour contaminated by 10(2) spores after 16 h of incubation.
Amaia González-Salgado; Teresa González-Jaén; Covadonga Vázquez; Belén Patiño
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment     Volume:  25     ISSN:  1944-0057     ISO Abbreviation:  Food Addit Contam Part A Chem Anal Control Expo Risk Assess     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-09-30     Revised Date:  2009-08-13    
Medline Journal Info:
Nlm Unique ID:  101485040     Medline TA:  Food Addit Contam Part A Chem Anal Control Expo Risk Assess     Country:  England    
Other Details:
Languages:  eng     Pagination:  758-64     Citation Subset:  IM    
Department of Genetics, Universidad Complutense de Madrid, Madrid 28040, Spain.
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MeSH Terms
Aspergillus flavus / classification,  isolation & purification*
DNA, Fungal / analysis
DNA, Ribosomal Spacer / analysis
Flour / microbiology*
Food Microbiology
Mycological Typing Techniques / methods
Polymerase Chain Reaction / methods
Triticum / microbiology*
Reg. No./Substance:
0/DNA, Fungal; 0/DNA, Ribosomal Spacer

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