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Highly selective preconcentration of ultra-trace cadmium by yeast surface engineering.
MedLine Citation:
PMID:  22846806     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The potential of selective cell-sorption for separation/preconcentration of ultra-trace heavy metals was exploited by surface engineering of Saccharomyces cerevisiae cells. The general idea is to display the cadmium-binding peptide on the cell surface in order to enhance the covalent interaction between cadmium and the yeast cells. By immobilizing the surface-engineered yeast cells onto cytopore(®) microcarrier beads for cadmium adsorption, we demonstrated that with respect to the native yeast 600-fold and 25-1000-fold improvements were observed respectively for the tolerance of ionic strength and the tolerant capability toward various metal cations after surface engineering. Based on these observations, a novel procedure for selective cadmium preconcentration was developed with detection by graphite furnace atomic absorption spectrometry (GFAAS), employing the engineered S. cerevisiae cell-loaded cytopore(®) beads as a renewable sorption medium incorporated into a sequential injection lab-on-valve system. The cadmium retained on the yeast cell surface was eluted with a small amount of nitric acid and quantified with GFAAS. Within a range of 5-100 ng L(-1) and a sample volume of 1 mL, an enrichment factor of 30 was achieved along with a detection limit of 1.1 ng L(-1), a sampling frequency of 20 h(-1) and a precision of 3.3% RSD at 50 ng L(-1). The procedure was validated by analyzing cadmium in certified reference materials and a series of environmental water samples.
Authors:
Ting Yang; Xiao-Xing Zhang; Ming-Li Chen; Jian-Hua Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-31
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Research Center for Analytical Sciences, College of Sciences, Box 332, Northeastern University, Shenyang 110819, China. chenml@mail.neu.edu.cn jianhuajrz@mail.neu.edu.cn.
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