| Highly selective preconcentration of ultra-trace cadmium by yeast surface engineering. | |
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MedLine Citation:
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PMID: 22846806 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The potential of selective cell-sorption for separation/preconcentration of ultra-trace heavy metals was exploited by surface engineering of Saccharomyces cerevisiae cells. The general idea is to display the cadmium-binding peptide on the cell surface in order to enhance the covalent interaction between cadmium and the yeast cells. By immobilizing the surface-engineered yeast cells onto cytopore(®) microcarrier beads for cadmium adsorption, we demonstrated that with respect to the native yeast 600-fold and 25-1000-fold improvements were observed respectively for the tolerance of ionic strength and the tolerant capability toward various metal cations after surface engineering. Based on these observations, a novel procedure for selective cadmium preconcentration was developed with detection by graphite furnace atomic absorption spectrometry (GFAAS), employing the engineered S. cerevisiae cell-loaded cytopore(®) beads as a renewable sorption medium incorporated into a sequential injection lab-on-valve system. The cadmium retained on the yeast cell surface was eluted with a small amount of nitric acid and quantified with GFAAS. Within a range of 5-100 ng L(-1) and a sample volume of 1 mL, an enrichment factor of 30 was achieved along with a detection limit of 1.1 ng L(-1), a sampling frequency of 20 h(-1) and a precision of 3.3% RSD at 50 ng L(-1). The procedure was validated by analyzing cadmium in certified reference materials and a series of environmental water samples. |
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Authors:
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Ting Yang; Xiao-Xing Zhang; Ming-Li Chen; Jian-Hua Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-31 |
Journal Detail:
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Title: The Analyst Volume: - ISSN: 1364-5528 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Research Center for Analytical Sciences, College of Sciences, Box 332, Northeastern University, Shenyang 110819, China. chenml@mail.neu.edu.cn jianhuajrz@mail.neu.edu.cn. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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