Document Detail

Highly selective microbial transformation of major ginsenoside Rb(1) to gypenoside LXXV by Esteya vermicola CNU120806.
MedLine Citation:
PMID:  22805203     Owner:  NLM     Status:  Publisher    
AIMS: This study examined the biotransformation pathway of ginsenoside Rb(1) by the fungus Esteya vermicola CNU 120806. METHODS AND RESULTS: Ginsenoside Rb(1) and Rd were extracted from the root of Panax ginseng. Liquid fermentation and purified enzyme hydrolysis were employed to investigate the biotransformation of ginsenoside Rb(1) . The metabolites were identified and confirmed using NMR analysis as gypenoside XVII and gypenoside LXXV. A mole yield of 95.4% gypenoside LXXV was obtained by enzymatic conversion (pH 5.0, temperature 50 °C). Ginsenoside Rd was used to verify the transformation pathway under the same reaction condition. The product Compound K (mole yield 49.6%) proved a consecutive hydrolyses occurred at the C-3 position of ginsenoside Rb(1) . CONCLUSIONS: Strain CNU 120806 showed a high degree of specific β-glucosidase activity to convert ginsenoside Rb(1) and Rd to gypenoside LXXV and Compound K respectively. The maximal activity of the purified glucosidase for ginsenosides transformation occurred at 50 °C and pH 5.0. Compared to its activity against pNPG (100%), the β-glucosidase exhibited quite lower level of activity against other aryl-glycosides. Enzymatic hydrolysate, gypenoside LXXV and Compound K were produced by consecutive hydrolyses of the terminal and inner glucopyranosyl moieties at the C-3 carbon of ginsenoside Rb(1) and Rd giving the pathway: ginsenoside Rb(1) → gypenoside XVII → gypenoside LXXV; ginsenoside Rd→F(2) →Compound K, but did not hydrolyze the 20-C, β-(1-6)-glucoside of ginsenoside Rb(1) and Rd. SIGNIFICANCE AND IMPACT OF THE STUDY: The results showed an important practical application on the preparation of gypenoside LXXV. Additionally, the present study for the first time provided a high efficient preparation method for gypenoside LXXV without further conversion, which also give rise to a potential commercial enzyme application. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Jin-Gang Hou; Jian-Jie Xue; Meng-Qi Sun; Chun-Yan Wang; Lei Liu; Dong-Liang Zhang; Mi-Ra Lee; Li-Juan Gu; Chun-Ling Wang; Yun-Bo Wang; Yin-An Zheng; Wei Li; Chang-Keun Sung
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-17
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Taejon, 305-764, Republic of Korea.
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