Document Detail


Highly purified lipoteichoic acid from gram-positive bacteria induces in vitro blood-brain barrier disruption through glia activation: role of pro-inflammatory cytokines and nitric oxide.
MedLine Citation:
PMID:  16343789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The co-culture of bovine brain capillary endothelial cells and rat primary glial cells was established as an in vitro blood-brain barrier model to investigate the mechanisms by which the Gram-positive bacterial cell wall components lipoteichoic acid and muramyl dipeptide induced injury of blood-brain barrier structure and function. We found that highly purified lipoteichoic acid disrupted blood-brain barrier integrity in a concentration- and time-dependent manner indirectly, through glia activation. Low trans-endothelial electrical resistance and high permeability to fluorescein isothiocyanate-inulin observed in the presence of lipoteichoic acid-activated glial cells were potentiated by muramyl dipeptide and could be reversed only when glial cells were activated by lipoteichoic acid at 10 microg/ml but not with a higher lipoteichoic acid concentration (30 microg/ml). Immunocytochemistry analysis revealed no evident changes in the distribution of the cytoskeleton protein F-actin and tight junction proteins occludin and claudin after lipoteichoic acid treatment. However, the tight junction associated protein AHNAK clearly revealed the morphological alteration of the endothelial cells induced by lipoteichoic acid. Lipoteichoic acid-activated glial cells produced nitric oxide and pro-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-1beta) that contributed to lipoteichoic acid-induced blood-brain barrier disruption, since the direct treatment of the endothelial monolayer with tumor necrosis factor-alpha or interleukin-1beta increased blood-brain barrier permeability, whereas the pre-treatment of lipoteichoic acid-activated glial cells with antibodies against these two cytokines blocked lipoteichoic acid effects. Additionally, nitric oxide was also involved in blood-brain barrier damage, since the nitric oxide donor itself (diethylenetriamine-nitric oxide adduct) increased blood-brain barrier permeability and inducible nitric oxide synthase inhibitor (1400W) partially reversed lipoteichoic acid-induced trans-endothelial electrical resistance decrease.
Authors:
M Boveri; A Kinsner; V Berezowski; A-M Lenfant; C Draing; R Cecchelli; M-P Dehouck; T Hartung; P Prieto; A Bal-Price
Related Documents :
47259 - Tissue specificity of nuclear acidic proteins isolated from bovine brain and adrenal me...
3036149 - Preparation of spin-labelled sulfatides for epr studies on model membranes.
6087629 - Beta-adrenergic influence on brain concentrations of monoamine precursors.
16525189 - Uptake and metabolism of plasma-derived erucic acid by rat brain.
17901539 - Brain uptake and utilization of fatty acids, lipids and lipoproteins: application to ne...
6428249 - Amino acid metabolism in the brain with convulsive disorders. part i: free amino acid p...
16730019 - Surface characterization by xps, contact angle measurements and tof-sims of cellulose f...
3840389 - Reutilization of surfactant phosphatidylcholine in adult rabbits.
11800229 - A new monoclonal antibody elisa for detection and characterization of c-telopeptide fra...
Publication Detail:
Type:  Journal Article     Date:  2005-12-15
Journal Detail:
Title:  Neuroscience     Volume:  137     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2006  
Date Detail:
Created Date:  2006-01-30     Completed Date:  2006-05-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1193-209     Citation Subset:  IM    
Affiliation:
European Centre for the Validation of Alternative Methods (ECVAM), Institute of Health and Consumer Protection, European Commission Joint Research Centre, Via E. Fermi 1, 21020 Ispra (VA), Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Blood-Brain Barrier / drug effects,  physiology*
Capillaries
Cattle
Cell Membrane Permeability
Cells, Cultured
Cerebral Cortex / blood supply*
Cytokines / physiology*
Cytoskeleton / drug effects,  physiology
Endothelium, Vascular / drug effects,  physiology*
Gram-Positive Bacteria / chemistry
Lipopolysaccharides / isolation & purification,  pharmacology*
Neuroglia / cytology,  drug effects,  physiology*
Nitric Oxide / physiology*
Teichoic Acids / isolation & purification,  pharmacology*
Tight Junctions / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Actins; 0/Cytokines; 0/Lipopolysaccharides; 0/Teichoic Acids; 10102-43-9/Nitric Oxide; 56411-57-5/lipoteichoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Investigating principles of human brain function underlying working memory: what insights from schiz...
Next Document:  Estrogen activates rapid signaling in the brain: role of estrogen receptor alpha and estrogen recept...