Document Detail


Highly efficient vitrification method for cryopreservation of human oocytes.
MedLine Citation:
PMID:  16176668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two experiments were performed to develop a method to cryopreserve MII human oocytes. In the first experiment, three vitrification methods were compared using bovine MII oocytes with regard to their developmental competence after cryopreservation: (i) vitrification within 0.25-ml plastic straws followed by in-straw dilution after warming (ISD method); (ii) vitrification in open-pulled straws (OPS method); and (iii) vitrification in <0.1 microl medium droplet on the surface of a specially constructed fine polypropylene strip attached to a plastic handle (Cryotop method). In the second experiment, the Cryotop method, which had yielded the best results, was used to vitrify human oocytes. Out of 64 vitrified oocytes, 58 (91%) exhibited normal morphology after warming. After intracytoplasmic sperm injection, 52 became fertilized, and 32 (50%) developed to the blastocyst stage in vitro. Analysis by fluorescence in-situ hybridization of five blastocysts showed that all were normal diploid embryos. Twenty-nine embryo transfers with a mean number of 2.2 embryos per transfer on days 2 and 5 resulted in 12 initial pregnancies, seven healthy babies and three ongoing pregnancies. The results suggest that vitrification using the Cryotop is the most efficient method for human oocyte cryopreservation.
Authors:
Masashige Kuwayama; Gábor Vajta; Osamu Kato; Stanley P Leibo
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  11     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-22     Completed Date:  2005-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  300-8     Citation Subset:  IM    
Affiliation:
Kato Ladies' Clinic, Tokyo, Japan. masaabc@bokkoame.ne.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / cytology,  physiology
Cattle
Cryopreservation / instrumentation,  methods*
Embryo Transfer
Ethylene Glycol / pharmacology
Female
Humans
In Situ Hybridization, Fluorescence
Male
Oocytes / drug effects,  physiology*
Pregnancy
Pregnancy Outcome
Pregnancy Rate
Sperm Injections, Intracytoplasmic
Chemical
Reg. No./Substance:
107-21-1/Ethylene Glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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