Document Detail

Highly efficient vitrification method for cryopreservation of human oocytes.
MedLine Citation:
PMID:  16176668     Owner:  NLM     Status:  MEDLINE    
Two experiments were performed to develop a method to cryopreserve MII human oocytes. In the first experiment, three vitrification methods were compared using bovine MII oocytes with regard to their developmental competence after cryopreservation: (i) vitrification within 0.25-ml plastic straws followed by in-straw dilution after warming (ISD method); (ii) vitrification in open-pulled straws (OPS method); and (iii) vitrification in <0.1 microl medium droplet on the surface of a specially constructed fine polypropylene strip attached to a plastic handle (Cryotop method). In the second experiment, the Cryotop method, which had yielded the best results, was used to vitrify human oocytes. Out of 64 vitrified oocytes, 58 (91%) exhibited normal morphology after warming. After intracytoplasmic sperm injection, 52 became fertilized, and 32 (50%) developed to the blastocyst stage in vitro. Analysis by fluorescence in-situ hybridization of five blastocysts showed that all were normal diploid embryos. Twenty-nine embryo transfers with a mean number of 2.2 embryos per transfer on days 2 and 5 resulted in 12 initial pregnancies, seven healthy babies and three ongoing pregnancies. The results suggest that vitrification using the Cryotop is the most efficient method for human oocyte cryopreservation.
Masashige Kuwayama; Gábor Vajta; Osamu Kato; Stanley P Leibo
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  11     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-22     Completed Date:  2005-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  300-8     Citation Subset:  IM    
Kato Ladies' Clinic, Tokyo, Japan.
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MeSH Terms
Blastocyst / cytology,  physiology
Cryopreservation / instrumentation,  methods*
Embryo Transfer
Ethylene Glycol / pharmacology
In Situ Hybridization, Fluorescence
Oocytes / drug effects,  physiology*
Pregnancy Outcome
Pregnancy Rate
Sperm Injections, Intracytoplasmic
Reg. No./Substance:
107-21-1/Ethylene Glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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