Document Detail

Highly efficient transport of carboxyfluorescein diacetate succinimidyl ester into COS7 cells using human papillomavirus-like particles.
MedLine Citation:
PMID:  12586349     Owner:  NLM     Status:  MEDLINE    
Human papillomavirus virus-like particles (VLPs) have recently been used to deliver genes into mammalian cells in vitro and in vivo. Here, we investigated whether VLPs may serve as an efficient carrier of low molecular weight compounds (e.g. hormones, vitamins, peptides etc.) into cells. COS7 cells were incubated with recombinant HPV-16L1/L2 VLPs labelled with the fluorescence dye carboxyfluorescein diacetate succinimidyl ester. Using flow cytometry, we demonstrate that labelled VLPs can specifically bind to the cell surface followed by their complete internalisation. Our results indicate that VLPs are promising vehicles for highly efficient delivery of low molecular weight compounds into cells.
Christian Bergsdorf; Carsten Beyer; Viktor Umansky; Margaret Werr; Martin Sapp
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  536     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-14     Completed Date:  2003-03-10     Revised Date:  2005-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  120-4     Citation Subset:  IM    
Virofem Diagnostica GmbH, Rheingaustrasse 190-196, P.O. Box 3540, 65174 Wiesbaden, Germany.
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MeSH Terms
Antibodies, Viral / pharmacology
Biological Transport
COS Cells
Capsid* / immunology,  metabolism
Capsid Proteins*
Fluoresceins / metabolism*
Fluorescent Dyes / metabolism*
Oncogene Proteins, Viral* / immunology,  isolation & purification,  metabolism
Succinimides / metabolism*
Virion / chemistry
Reg. No./Substance:
0/5-(6)-carboxyfluorescein diacetate succinimidyl ester; 0/Antibodies, Viral; 0/Capsid Proteins; 0/Fluoresceins; 0/Fluorescent Dyes; 0/L1 protein, Human papillomavirus type 16; 0/L2 protein, Human papillomavirus type 16; 0/Oncogene Proteins, Viral; 0/Succinimides; 0/Virosomes

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