Document Detail


Highly differential expression of SN1, a bidirectional glutamine transporter, in astroglia and endothelium in the developing rat brain.
MedLine Citation:
PMID:  12528181     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transmitters glutamate and GABA also subserve trophic action and are required for normal development of the brain. They are formed from glutamine, which may be synthesized in glia or extracted from the blood. In the adult, the glutamine transporter SN1 is expressed in the astroglia. SN1 works in both directions, depending on the concentration gradients of its substrates and cotransported ions, and is thought to regulate extracellular glutamine and to supply the neurons with the transmitter precursor. In this article, we have quantified the expression and studied the localization of SN1 at different developmental stages. SN1 is expressed in astroglia throughout the CNS from embryonic stages through adulthood. No indication of SN1 staining in neuronal elements has been obtained at any stage. Quantitative immunoblotting of whole brain extracts demonstrates increasing expression of SN1 from P0, reaching a peak at P14, twice the adult level. A moderate and slower rise and fall of the expression levels of SN1 occurs in the cerebellum. Strong transient SN1-like staining is also found in Bergmann glia and vascular endothelium in the first postnatal weeks. Strong intracellular staining in the same time period suggests a high rate of SN1 synthesis in the early postnatal period. This coincides with the increasing levels of glutamate and GABA in the CNS and with the time course of synaptogenesis. This study suggests that the expression of SN1 is highly regulated, correlating with the demand for glutamine during the critical period of development.
Authors:
Jean-Luc Boulland; Amina Rafiki; Line M Levy; Jon Storm-Mathisen; Farrukh A Chaudhry
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glia     Volume:  41     ISSN:  0894-1491     ISO Abbreviation:  Glia     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-01-15     Completed Date:  2003-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  260-75     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Affiliation:
Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Amino Acid Transport Systems, Neutral*
Animals
Antibody Specificity
Astrocytes / metabolism*,  ultrastructure
Blood-Brain Barrier / physiology*
Brain / cytology,  growth & development*,  metabolism*
Endothelium, Vascular / cytology,  metabolism
Female
Male
Membrane Transport Proteins / biosynthesis*,  immunology,  metabolism
Microscopy, Electron
Pregnancy
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Amino Acid Transport Systems, Neutral; 0/Membrane Transport Proteins; 0/system N protein 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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