Document Detail

Highly confined surface imaging by solid immersion total internal reflection fluorescence microscopy.
MedLine Citation:
PMID:  22330569     Owner:  NLM     Status:  In-Data-Review    
We report the use of a high-refractive-index aplanatic solid immersion lens (ASIL) in total internal reflection fluorescence (TIRF) microscopy. This new solid immersion total internal reflection fluorescence (SITIRF) microscopy allows highly confined surface imaging with a significantly reduced imaging depth compared with conventional TIRF microscopy. We explore the application of a high refractive index, low optical dispersion material zirconium dioxide in the SITIRF microscope and also introduce a novel system design which enables the SITIRF microscope to work either in the epi-fluorescence or TIRF modes with variable illumination angles. We use both synthetic and biological samples to demonstrate that the imaging depth in the SITIRF microscope can be confined to a few tens of nanometers. SITIRF microscopy has the advantages of performing highly selective imaging and high-resolution high-contrast imaging. Potential applications in biological imaging and future developments of SITIRF microscopy are proposed.
Lin Wang; Cvetelin Vasilev; Daniel P Canniffe; Luke R Wilson; C Neil Hunter; Ashley J Cadby
Related Documents :
18270109 - Vision processing for realtime 3-d data acquisition based on coded structured light.
20234339 - Tomato analyzer: a useful software application to collect accurate and detailed morphol...
7896949 - A rudimentary system for automatic discrimination among basic skin lesions on the basis...
17792379 - Lunar impact basins and crustal heterogeneity: new western limb and far side data from ...
2339829 - Comparison of color flow and 3d image by computer graphics for the evaluation of caroti...
12644039 - Evaluation of hemodynamics in esophageal varices. value of endoscopic color doppler ult...
7644329 - 3-d shape perception.
10766059 - Computer-assisted spine surgery.
21951009 - Diffusion tensor imaging the medial longitudinal fasciculus in ino: opportunities and c...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Optics express     Volume:  20     ISSN:  1094-4087     ISO Abbreviation:  Opt Express     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-02-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137103     Medline TA:  Opt Express     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3311-24     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Transient behaviors of current-injection quantum-dot microdisk lasers.
Next Document:  Compact in-line autocorrelator using double wedge.