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Highly Sensitive Measurement of Whole Blood Chromium by Inductively Coupled Plasma Mass Spectrometry.
MedLine Citation:
PMID:  23127384     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVES: Chromium (Cr), a trace metal element, is implicated in diabetes and cardiovascular disease. A hypochromic state has been associated with poor blood glucose control and unfavorable lipid metabolism. Sensitive and accurate measurement of blood chromium is very important to assess the chromium nutritional status. However, interferents in biological matrices and contamination make the sensitive analysis challenging. The primary goal of this study was to develop a highly sensitive method for quantification of total Cr in whole blood by inductively coupled plasma mass spectrometry (ICP-MS) and to validate the reference interval in a local healthy population. DESIGN AND METHODS: This method was developed on an ICP-MS with a collision/reaction cell. Interference was minimized using both kinetic energy discrimination between the quadrupole and hexapole and a selective collision gas (helium). Reference interval was validated in whole blood samples (n=51) collected in trace element free EDTA tubes from healthy adults (12 males, 39 females), aged 19-64 y (38.8±12.6), after a minimum of 8h fasting. Blood samples were aliquoted into cryogenic vials and stored at -70°C until analysis. RESULTS: The assay linearity was 3.42 to 1446.59nmol/L with an accuracy of 87.7 to 99.8%. The high sensitivity was achieved by minimization of interference through selective kinetic energy discrimination and selective collision using helium. The reference interval for total Cr using a non-parametric method was verified to be 3.92 to 7.48nmol/L. CONCLUSION: This validated ICP-MS methodology is highly sensitive and selective for measuring total Cr in whole blood.
Authors:
Wendy Cieslak; Kathleen Pap; Dustin R Bunch; Edmunds Reineks; Raymond Jackson; Roxanne Steinle; Sihe Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-2
Journal Detail:
Title:  Clinical biochemistry     Volume:  -     ISSN:  1873-2933     ISO Abbreviation:  Clin. Biochem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA.
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