| A Highly Sensitive, Dual-Readout Assay Based on Gold Nanoparticles for Organophosphorus and Carbamate Pesticides. | |
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MedLine Citation:
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PMID: 22475016 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The report presents a highly sensitive, Rhodamine B-covered gold nanoparticle (RB-AuNP)-based assay with dual readouts (colorimetric and fluorometric) for detecting organophosphorus and carbamate pesticides in complex solutions. The detection mechanism is based on the fact that these pesticides can inhibit the activity of acetylcholinesterase (AChE), thus preventing the generation of thiocholine (which turns the RB-AuNPs solutions blue and unquenches the fluorescence of RB simultaneously). The color of the RB-AuNPs solution remains red and the fluorescence of RB remains quenched. Using this dual-readout assay, the lowest detectable concentrations for several kinds of pesticides including Carbaryl, Diazinon, Malathion, and Phorate were measured to be 0.1μg/L, 0.1 μg/L, 0.3 μg/L and 1 μg/L, respectively, all of which are much lower than the maximum residue limits (MRL) as reported in the European Union pesticides database as well as those from United States Department Agriculture (USDA). This assay allows detection of pesticides in real samples such as agricultural products and river water. The results in detecting pesticide residues collected from food samples using this method agree well with those from high performance liquid chromatography (HPLC). This simple assay is therefore suitable for sensing pesticides in complex samples especially in combination with other portable platforms. |
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Authors:
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Dingbin Liu; Wenwen Chen; Jinhua Wei; Xuebing Li; Zhuo Wang; Xingyu Jiang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-5 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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