Document Detail

A highly efficient Ziehl-Neelsen stain: identifying de novo intracellular Mycobacterium tuberculosis and improving detection of extracellular M. tuberculosis in cerebrospinal fluid.
MedLine Citation:
PMID:  22238448     Owner:  NLM     Status:  MEDLINE    
Tuberculous meningitis leads to a devastating outcome, and early diagnosis and rapid chemotherapy are vital to reduce morbidity and mortality. Since Mycobacterium tuberculosis is a kind of cytozoic pathogen and its numbers are very few in cerebrospinal fluid, detecting M. tuberculosis in cerebrospinal fluid from tuberculous meningitis patients is still a challenge for clinicians. Ziehl-Neelsen stain, the current feasible microbiological method for the diagnosis of tuberculosis, often needs a large amount of cerebrospinal fluid specimen but shows a low detection rate of M. tuberculosis. Here, we developed a modified Ziehl-Neelsen stain, involving cytospin slides with Triton processing, in which only 0.5 ml of cerebrospinal fluid specimens was required. This method not only improved the detection rate of extracellular M. tuberculosis significantly but also identified intracellular M. tuberculosis in the neutrophils, monocytes, and lymphocytes clearly. Thus, our modified method is more effective and sensitive than the conventional Ziehl-Neelsen stain, providing clinicians a convenient yet powerful tool for rapidly diagnosing tuberculous meningitis.
Ping Chen; Ming Shi; Guo-Dong Feng; Jia-Yun Liu; Bing-Ju Wang; Xiao-Dan Shi; Lei Ma; Xue-Dong Liu; Yi-Ning Yang; Wen Dai; Ting-Ting Liu; Ying He; Jin-Ge Li; Xiao-Ke Hao; Gang Zhao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-11
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-19     Completed Date:  2012-07-10     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1166-70     Citation Subset:  IM    
Department of Neurology, Xijing Hospital, the Fourth Military Medical University, Xi’an, Shaanxi 710032, China.
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MeSH Terms
Coloring Agents / chemistry*
Lymphocytes / microbiology
Mycobacterium tuberculosis*
Neutrophils / microbiology
Sensitivity and Specificity
Staining and Labeling / methods*
Tuberculosis, Meningeal / cerebrospinal fluid*,  diagnosis*,  microbiology
Reg. No./Substance:
0/Coloring Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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