| Higher sperm DNA damage in semen from men with spinal cord injuries compared with controls. | |
| | |
MedLine Citation:
|
PMID: 17804864 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Semen from men with spinal cord injuries (SCI) and control subjects was investigated for sperm DNA damage using the sperm chromatin structure assay. Three experiments were performed. In experiment 1, the DNA fragmentation index (DFI) was compared in semen from SCI subjects and control subjects. In experiment 2, the % DFI was determined in repeated ejaculations to examine the effect of anejaculation on DFI. In experiment 3, the DFI was determined in neat vs processed semen to examine the effect of necrospermia or leukocytospermia on DFI. The results of experiment 1 showed a significantly higher mean (+/- SEM) DFI in the semen of SCI subjects (65.2% +/- 6.6%; range, 42.3%-90.8%) compared with control subjects (15.4% +/- 2.9%; range, 5.4%-33.5%; P < .001). In experiment 2, there was a high correlation between the DFIs obtained in the first semen specimens and the DFIs obtained 3 days later in semen of the same SCI subjects (r(s) = .94; P < .02). In experiment 3, the results showed no significant difference between mean DFI in aliquots of neat semen (79.3% +/- 9.9%) vs matched aliquots of semen processed to remove dead sperm and leukocytes in SCI subjects (75.2% +/- 16.1%). The DFI is higher in semen from men with SCI vs controls. The cause of this condition is unknown but does not seem to be due to prolonged anejaculation or to the proximate conditions of necrospermia or leukocytospermia. The relevance of these findings to fertility outcomes with SCI male partners remains to be determined. |
| | |
Authors:
|
Nancy L Brackett; Emad Ibrahim; Jason A Grotas; Teodoro C Aballa; Charles M Lynne |
Related Documents
:
|
23670874 - Modeling the effects of immunizations timing on child health outcomes in india. 21743294 - Informed consent in vaccination in india: medicolegal aspects. 6505784 - Experimental infection of oncomelania quadrasi with paragonimus ohirai. 23571224 - Rabies pre-exposure vaccination of children with purified chick embryo cell vaccine (pc... 3020494 - Varicella vaccine studies in healthy children and adults. 11227884 - Redescription and systematic status of capillaria philippinensis, an intestinal parasit... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-05 |
Journal Detail:
|
Title: Journal of andrology Volume: 29 ISSN: 0196-3635 ISO Abbreviation: J. Androl. Publication Date: 2008 Jan-Feb |
Date Detail:
|
Created Date: 2007-12-24 Completed Date: 2008-03-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8106453 Medline TA: J Androl Country: United States |
Other Details:
|
Languages: eng Pagination: 93-9; discussion 100-1 Citation Subset: IM |
Affiliation:
|
Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Lois Pope Life Center, 1095 NW 14th Terrace, Miami, FL 33136, USA. nbrackett@miami.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Cell Death Chromatin / pathology DNA Damage* Ejaculation Humans Infertility, Male / etiology*, pathology* Leukocytes Male Middle Aged Spermatozoa / pathology* Spinal Cord Injuries / complications* |
| Chemical | |
Reg. No./Substance:
|
0/Chromatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Tissue distribution and metabolism of benzo[a]pyrene in embryonic and larval medaka (Oryzias latipes...
Next Document: Proinflammatory cytokines as an intermediate factor enhancing lipid sperm membrane peroxidation in i...