| Higher-order nuclear organization in growth arrest of human mammary epithelial cells: a novel role for telomere-associated protein TIN2. | |
| | |
MedLine Citation:
|
PMID: 15741234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Nuclear organization, such as the formation of specific nuclear subdomains, is generally thought to be involved in the control of cellular phenotype; however, there are relatively few specific examples of how mammalian nuclei organize during radical changes in phenotype, such as those occurring during differentiation and growth arrest. Using human mammary epithelial cells in which growth arrest is essential for morphological differentiation, we show that the arrest of cell proliferation is accompanied by a reorganization of the telomere-associated protein, TIN2, into one to three large nuclear subdomains. The large TIN2 domains do not contain telomeres and occur concomitant with the continued presence of TIN2 at telomeres. The TIN2 domains were sensitive to DNase, but not RNase, occurred frequently, but not exclusively near nucleoli, and overlapped often with dense domains containing heterochromatin protein 1gamma. Expression of truncated forms of TIN2 simultaneously prevented the formation of TIN2 domains and relaxed the stringent morphogenesis-induced growth arrest in human mammary epithelial cells. Here we show that a novel extra-telomeric organization of TIN2 is associated with the control of cell proliferation and identify TIN2 as an important regulator of mammary epithelial differentiation. |
| | |
Authors:
|
Patrick Kaminker; Cedric Plachot; Sahn-Ho Kim; Peter Chung; Danielle Crippen; Ole W Petersen; Mina J Bissell; Judith Campisi; Sophie A Lelièvre |
Related Documents
:
|
20965244 - Honeybee trophocytes and fat cells as target cells for cellular senescence studies. 20525204 - Senescence evasion by mcf-7 human breast tumor-initiating cells. 15126344 - Role of the retinoblastoma pathway in senescence triggered by repression of the human p... 10498864 - Telomere shortening triggers a p53-dependent cell cycle arrest via accumulation of g-ri... 20965244 - Honeybee trophocytes and fat cells as target cells for cellular senescence studies. 16376114 - Anion-exchange blocker enhances cytoplasmic vacuole formation and cell death in serum-d... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. Date: 2005-03-01 |
Journal Detail:
|
Title: Journal of cell science Volume: 118 ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 2005 Mar |
Date Detail:
|
Created Date: 2005-03-14 Completed Date: 2005-09-15 Revised Date: 2011-08-01 |
Medline Journal Info:
|
Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: England |
Other Details:
|
Languages: eng Pagination: 1321-30 Citation Subset: IM |
Affiliation:
|
Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, California 94945, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blotting, Western Breast / cytology* Bromodeoxyuridine / pharmacology Cell Culture Techniques / methods Cell Differentiation Cell Nucleus / metabolism* Cell Proliferation Cells, Cultured Deoxyribonuclease I / metabolism Deoxyribonucleases / metabolism Epithelial Cells / cytology*, metabolism Heterochromatin / chemistry Humans Immunohistochemistry In Situ Hybridization, Fluorescence Ki-67 Antigen / biosynthesis Microscopy, Fluorescence Phenotype Protein Structure, Tertiary Retroviridae / genetics Ribonuclease, Pancreatic / metabolism Ribonucleases / metabolism Telomere / metabolism, ultrastructure Telomere-Binding Proteins / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
AG00266/AG/NIA NIH HHS; AG09909/AG/NIA NIH HHS; CA64786/CA/NCI NIH HHS; R01 CA064786-05/CA/NCI NIH HHS; R37 AG009909-10/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Heterochromatin; 0/Ki-67 Antigen; 0/TINF2 protein, human; 0/Telomere-Binding Proteins; 59-14-3/Bromodeoxyuridine; EC 3.1.-/Deoxyribonucleases; EC 3.1.-/Ribonucleases; EC 3.1.21.1/Deoxyribonuclease I; EC 3.1.27.5/Ribonuclease, Pancreatic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A dynamin-3 spliced variant modulates the actin/cortactin-dependent morphogenesis of dendritic spine...
Next Document: The neurogene BTG2TIS21/PC3 is transactivated by DeltaNp73alpha via p53 specifically in neuroblastom...