Document Detail


Higher accumulation of gamma-aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidases in Glycine max (L.) Merr. roots.
MedLine Citation:
PMID:  17624796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyamines (PAs) are assumed to perform their functions through their oxidative product such as gamma-aminobutyric acid (GABA) formation. However, there is only limited information on the interrelation between PA degradation and GABA accumulation under salt stress. In order to reveal a quantitative correlation between PA oxidation and GABA accumulation, the effects of treatments with different NaCl concentrations, along with aminoguanidine (AG, a specific inhibitor of diamine oxidases (DAO; EC: 1.4.3.6)) and a recovery test from salt stress on endogenous free PAs, gamma-aminobutyric acid (GABA) accumulation and DAO activity were determined in roots of soybean [Glycine max (L.) Merr.] cultivar Suxie-1. The results showed that the levels of putrescine (Put), cadaverine (Cad), and spermidine (Spd) decreased significantly with increasing salt concentrations. This occurred because salt stress strongly promoted DAO activity to stimulate PA degradation. GABA accumulation increased with growing NaCl concentrations, about an 11- to 17-fold increase as compared with the control plants. AG treatment increased the accumulation of endogenous free PAs as a result of a strong retardation of DAO activity, but decreased GABA accumulation. The recovery for 6 days in 1/2 Hoagland solution from 100mM NaCl stress resulted in a decrease in DAO activity, a rebound of PA levels and a simultaneous reduction of GABA content. A close correlation was observed between the changes in DAO activity and GABA accumulation. The results indicated that higher GABA accumulation (about 39%) induced by salt stress could come from PA degradation, suggesting that PAs might perform their functions through GABA formation under salt stress.
Authors:
Su Guo Xing; Yu Bing Jun; Zhang Wen Hau; Liu You Liang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-06
Journal Detail:
Title:  Plant physiology and biochemistry : PPB / Société française de physiologie végétale     Volume:  45     ISSN:  0981-9428     ISO Abbreviation:  Plant Physiol. Biochem.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-01     Completed Date:  2007-12-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9882449     Medline TA:  Plant Physiol Biochem     Country:  France    
Other Details:
Languages:  eng     Pagination:  560-6     Citation Subset:  IM    
Affiliation:
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Amine Oxidase (Copper-Containing) / chemistry,  metabolism*
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Guanidines / chemistry
Oxygen / metabolism
Plant Physiological Phenomena
Plant Roots / drug effects,  metabolism*
Polyamines / chemistry*
Salts / pharmacology*
Sodium Chloride / chemistry
Soybeans / metabolism
Time Factors
gamma-Aminobutyric Acid / biosynthesis*
Chemical
Reg. No./Substance:
0/Guanidines; 0/Polyamines; 0/Salts; 56-12-2/gamma-Aminobutyric Acid; 7647-14-5/Sodium Chloride; 7782-44-7/Oxygen; 79-17-4/pimagedine; EC 1.4.3.6/Amine Oxidase (Copper-Containing)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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