Document Detail

Higher DNA repair activity is related with longer replicative life span in mammalian embryonic fibroblast cells.
MedLine Citation:
PMID:  21879286     Owner:  NLM     Status:  MEDLINE    
Since the detailed comparison of DNA repair activities among mammalian embryonic fibroblast cells with different replicative life spans has not been investigated, we tested DNA repair activities in embryonic fibroblast cells derived from mammals including human, dog, rat, and mouse. The cell viability after treatment of four DNA damage agents appeared to be decreased in the order of human embryonic fibroblasts (HEFs) > dog embryonic fibroblasts (DEFs) > rat embryonic fibroblasts (REFs) > mouse embryonic fibroblasts (MEFs) although statistical significance was lacking. The amounts of strand breaks and AP (apurinic/apyrimidinic) sites also appear to be decreased in the order of HEFs > DEFs > REFs ≥ MEFs after treatment of DNA damage agents. The DNA repair activities and rates including base excision repair (BER), nucleotide excision repair (NER) and double-strand break repair (DSBR) including non-homologous end-joining (NHEJ) decreased again in the order of HEFs > DEFs > REFs ≥ MEFs. BER and NHEJ activities in 3% O(2) also decreased in the order of HEFs > DEFs > REFs > MEFs. This order in DNA repair activity appears to be coincident with that of replicative life span of fibroblasts and that of life span of mammals. These results indicate that higher DNA repair activity is related with longer replicative life span in embryonic fibroblast cells.
Seong-Hoon Park; Hong-Jun Kang; Hyun-Seok Kim; Min-Ju Kim; Jee-In Heo; Jeong-Hyeon Kim; Yoon-Jung Kho; Sung Chan Kim; Jaebong Kim; Jae-Bong Park; Jae-Yong Lee
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-31
Journal Detail:
Title:  Biogerontology     Volume:  12     ISSN:  1573-6768     ISO Abbreviation:  Biogerontology     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-04     Completed Date:  2012-02-23     Revised Date:  2013-04-24    
Medline Journal Info:
Nlm Unique ID:  100930043     Medline TA:  Biogerontology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  565-79     Citation Subset:  IM    
Department of Radiation Oncology, Vanderbilt University, Nashville, TN 37232, USA.
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MeSH Terms
Camptothecin / pharmacology
Cell Aging* / drug effects
Cell Proliferation* / drug effects
Cell Survival
Cells, Cultured
Cisplatin / pharmacology
DNA Damage*
DNA Repair* / drug effects
Dose-Response Relationship, Drug
Embryo, Mammalian / cytology
Etoposide / pharmacology
Fibroblasts / drug effects,  metabolism*
Hydrogen Peroxide / pharmacology
Mice, Inbred ICR
Rats, Sprague-Dawley
Reg. No./Substance:
15663-27-1/Cisplatin; 33419-42-0/Etoposide; 7689-03-4/Camptothecin; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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