| High-yield purification of rat monocytes by combined density gradient and immunomagnetic separation. | |
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MedLine Citation:
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PMID: 8613672 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Satisfactory purification of rodent monocytes in suspension has not been achieved up to now because in rats and mice these cells occur as a minor population of peripheral blood leukocytes overlapping with lymphocytes in size and density. We describe a two-step procedure for the isolation of monocytes from rat blood with high yield and purity. This method permits the recovery of more than 90% of monocytes collected by perfusion of the vasculature and avoids loss of major subpopulations. Percoll density gradient centrifugation of perfusate cells is combined with subsequent indirect immunomagnetic depletion of lymphocytes using an antibody cocktail. The method described produces more than 90% pure rat monocytes. |
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Authors:
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A Scriba; L Luciano; B Steiniger |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunological methods Volume: 189 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-06-06 Completed Date: 1996-06-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 203-16 Citation Subset: IM |
Affiliation:
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Institute of Anatomy and Cell Biology, University of Marburg, D-35037 Marburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Separation / methods* Centrifugation, Density Gradient Immunomagnetic Separation* Immunophenotyping Male Mice Monocytes* Perfusion Rats Rats, Inbred Lew |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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