| High yield production of d-xylonic acid from d-xylose using engineered Escherichia coli. | |
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MedLine Citation:
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PMID: 21917451 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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An engineered Escherichia coli was constructed to produce d-xylonic acid, one of the top 30 high-value chemicals identified by US Department of Energy. The native pathway for d-xylose catabolism in E. coli W3110 was blocked by disrupting xylose isomerase (XI) and xylulose kinase (XK) genes. The native pathway for xylonic acid catabolism was also blocked by disrupting two genes both encoding xylonic acid dehydratase (yagE and yjhG). Through the introduction of a d-xylose dehydrogenase from Caulobacter crescentus, a d-xylonic acid producing E. coli was constructed. The recombinant E. coli produced up to 39.2gL(-1)d-xylonic acid from 40gL(-1)d-xylose in M9 minimal medium. The average productivity was as high as 1.09gL(-1)h(-1) and no gluconic acid byproduct was produced. These results suggest that the engineered E. coli has a promising application for the industrial-scale production of d-xylonic acid. |
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Authors:
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Huaiwei Liu; Kris Niño G Valdehuesa; Grace M Nisola; Kristine Rose M Ramos; Wook-Jin Chung |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-22 |
Journal Detail:
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Title: Bioresource technology Volume: - ISSN: 1873-2976 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-9-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Energy and Environment Fusion Technology Center (E(2)FTC), Myongji University, Yongin City, Gyeonggi Province 449-728, South Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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