| High-yield method for dispersing simian kidneys for cell cultures. | |
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MedLine Citation:
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PMID: 4993235 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A technique for dispersion of animal tissue cells is described. The proposed technique is based on the concomitant use of trypsin and disodium ethylenediamine tetraacetate (EDTA). The use of the two dispersing agents (trypsin and disodium EDTA) markedly enhances cell yield as compared with the standard cell dispersion methods. Moreover, significant reduction in the amount of time required for complete tissue dispersal, presence of a very low number of nonviable cells, less cell clumping, and more uniform monolayer formation upon cultivation compare favorably with the results usually obtained with the standard trypsinization technique. |
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Authors:
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H M de Oca; P Probst; R Grubbs |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied microbiology Volume: 21 ISSN: 0003-6919 ISO Abbreviation: Appl Microbiol Publication Date: 1971 Jan |
Date Detail:
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Created Date: 1971-03-12 Completed Date: 1971-03-12 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 7605802 Medline TA: Appl Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 90-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Count Cricetinae Culture Media Culture Techniques* Edetic Acid Haplorhini Kidney Methods Mice Rabbits Rats Tissue Extracts Trypsin |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Tissue Extracts; 60-00-4/Edetic Acid; EC 3.4.21.4/Trypsin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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