Document Detail


High-yield method for dispersing simian kidneys for cell cultures.
MedLine Citation:
PMID:  4993235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A technique for dispersion of animal tissue cells is described. The proposed technique is based on the concomitant use of trypsin and disodium ethylenediamine tetraacetate (EDTA). The use of the two dispersing agents (trypsin and disodium EDTA) markedly enhances cell yield as compared with the standard cell dispersion methods. Moreover, significant reduction in the amount of time required for complete tissue dispersal, presence of a very low number of nonviable cells, less cell clumping, and more uniform monolayer formation upon cultivation compare favorably with the results usually obtained with the standard trypsinization technique.
Authors:
H M de Oca; P Probst; R Grubbs
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  21     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1971 Jan 
Date Detail:
Created Date:  1971-03-12     Completed Date:  1971-03-12     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  90-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count
Cricetinae
Culture Media
Culture Techniques*
Edetic Acid
Haplorhini
Kidney
Methods
Mice
Rabbits
Rats
Tissue Extracts
Trypsin
Chemical
Reg. No./Substance:
0/Culture Media; 0/Tissue Extracts; 60-00-4/Edetic Acid; EC 3.4.21.4/Trypsin
Comments/Corrections

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