| High-titer production of monomeric hydroxyvalerates from levulinic acid in Pseudomonas putida. | |
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MedLine Citation:
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PMID: 18938201 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hydroxyacids represent an important class of compounds that see application in the production of polyesters, biodegradable plastics and antibiotics, and that serve as useful chiral synthetic building blocks for other fine chemicals and pharmaceuticals. An economical, high-titer method for the production of 4-hydroxyvalerate (4HV) and 3-hydroxyvalerate (3HV) from the inexpensive and renewable carbon source levulinic acid was developed. These hydroxyvalerates were produced by periodically feeding levulinate to Pseudomonas putida KT2440 expressing a recombinant thioesterase II (tesB) gene from Escherichia coli K12. The titer of 4HV in shake flask culture reached 13.9+/-1.2 g L(-1) from P. putida tesB(+) cultured at 32 degrees C in LB medium periodically supplemented with glucose and levulinate. The highest 3HV titer obtained was 5.3+/-0.1 g L(-1) in M9 minimal medium supplemented with glucose and levulinate. |
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Authors:
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Collin H Martin; Kristala L Jones Prather |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-25 |
Journal Detail:
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Title: Journal of biotechnology Volume: 139 ISSN: 0168-1656 ISO Abbreviation: J. Biotechnol. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-05-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8411927 Medline TA: J Biotechnol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 61-7 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism Escherichia coli K12 / enzymology, genetics Fatty Acid Synthetase Complex / genetics, metabolism Kinetics Levulinic Acids / metabolism* Pentanoic Acids / analysis, metabolism* Pseudomonas putida / genetics, metabolism* Recombinant Proteins / genetics, metabolism Thiolester Hydrolases / genetics, metabolism Valerates / analysis, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Levulinic Acids; 0/Pentanoic Acids; 0/Recombinant Proteins; 0/Valerates; 10237-77-1/beta-hydroxyvaleric acid; 123-76-2/levulinic acid; 13532-37-1/4-hydroxyvaleric acid; EC 3.1.2.-/Thiolester Hydrolases; EC 3.1.2.-/thioesterase II; EC 6.-/Fatty Acid Synthetase Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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