Document Detail


High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol.
MedLine Citation:
PMID:  18336209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reversely transfected cell microarrays (RTCM) have been introduced as a method for parallel high throughput analysis of gene functions in mammalian cells. Hundreds to thousands of different recombinant DNA or RNA molecules can be transfected into different cell clusters at the same time on a single glass slide with this method. This allows either the simultaneous overexpression or--by using the recently developed RNA interference (RNAi) techniques--knockdown of a huge number of target genes. A growing number of sophisticated detection systems have been established to determine quantitatively the effects of the transfected molecules on the cell phenotype. Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol.
Authors:
Michael Stürzl; Andreas Konrad; Gaby Sander; Effi Wies; Frank Neipel; Elisabeth Naschberger; Simone Reipschläger; Nathalie Gonin-Laurent; Raymund E Horch; Ulrich Kneser; Werner Hohenberger; Holger Erfle; Mathias Thurau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Combinatorial chemistry & high throughput screening     Volume:  11     ISSN:  1386-2073     ISO Abbreviation:  Comb. Chem. High Throughput Screen.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-03-13     Completed Date:  2008-05-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810948     Medline TA:  Comb Chem High Throughput Screen     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  159-72     Citation Subset:  IM    
Affiliation:
Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Schwabachanlage 10, D-91054 Erlangen, Germany. michael.stuerzl@uk-erlangen.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Drug Evaluation, Preclinical / methods*
Gene Expression Regulation / genetics,  physiology
Gene Silencing
Genes / genetics*,  physiology*
Humans
Reverse Transcription / genetics*
Tissue Array Analysis / methods*
Transfection / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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