Document Detail


High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol.
MedLine Citation:
PMID:  18336209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reversely transfected cell microarrays (RTCM) have been introduced as a method for parallel high throughput analysis of gene functions in mammalian cells. Hundreds to thousands of different recombinant DNA or RNA molecules can be transfected into different cell clusters at the same time on a single glass slide with this method. This allows either the simultaneous overexpression or--by using the recently developed RNA interference (RNAi) techniques--knockdown of a huge number of target genes. A growing number of sophisticated detection systems have been established to determine quantitatively the effects of the transfected molecules on the cell phenotype. Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol.
Authors:
Michael Stürzl; Andreas Konrad; Gaby Sander; Effi Wies; Frank Neipel; Elisabeth Naschberger; Simone Reipschläger; Nathalie Gonin-Laurent; Raymund E Horch; Ulrich Kneser; Werner Hohenberger; Holger Erfle; Mathias Thurau
Related Documents :
15245899 - Cationic lipid emulsions containing heavy oils for the transfection of adherent cells.
11833069 - Vegf165 antisense rna suppresses oncogenic properties of human esophageal squamous cell...
25187829 - Emodin inhibits lovo colorectal cancer cell proliferation via the regulation of the bcl...
3015989 - Transformation of diploid human fibroblasts by dna transfection with the v-sis oncogene.
19815039 - How to study dendriplexes ii: transfection and cytotoxicity.
10340929 - Restoration of cyclic adenosine monophosphate-stimulated chloride channel activity in h...
24561519 - Bim upregulation and ros-dependent necroptosis mediate the antitumor effects of the hda...
9169049 - Fibroblast growth factor receptor-1 (fgfr-1) is essential for normal neural tube and li...
22065859 - Follicular assembly: mechanisms of action.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Combinatorial chemistry & high throughput screening     Volume:  11     ISSN:  1386-2073     ISO Abbreviation:  Comb. Chem. High Throughput Screen.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-03-13     Completed Date:  2008-05-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810948     Medline TA:  Comb Chem High Throughput Screen     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  159-72     Citation Subset:  IM    
Affiliation:
Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Schwabachanlage 10, D-91054 Erlangen, Germany. michael.stuerzl@uk-erlangen.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Drug Evaluation, Preclinical / methods*
Gene Expression Regulation / genetics,  physiology
Gene Silencing
Genes / genetics*,  physiology*
Humans
Reverse Transcription / genetics*
Tissue Array Analysis / methods*
Transfection / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Rational design, selection and specificity of artificial transcription factors (ATFs): the influence...
Next Document:  Discovery of novel targets with high throughput RNA interference screening.