| High-throughput screening of dioxins in sediment and soil using selective pressurized liquid extraction with immunochemical detection. | |
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MedLine Citation:
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PMID: 19818991 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A high-throughput screening method using selective pressurized liquid extraction (SPLE) and enzyme-linked immunosorbent assay (ELISA) for monitoring dioxins in sediment and soil is described. SPLE conditions were developed by extracting sediment or soil together with alumina, 10% AgNO3 in silica, and sulfuric acid impregnated silica (acid silica) using dichloromethane (DCM) as the solvent at 100 degrees C and 2000 psi. Post-extraction cleanups were not required for ELISA. Two reference sediments (National Institute of Standards and Technology SRM 1944 and Wellington Laboratories WMS01) were analyzed by the SPLE-ELISA method. The ELISA utilized a polyclonal antibody and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as the calibrant. Recoveries of ELISA-derived TCDD equivalents (EQ) relative to the expected gas chromatography/high resolution mass spectrometry (GC/HRMS) derived dioxin toxic equivalent (TEQ) values were 116+/-11% for SRM 1944 and 102+/-13% for WMS01. ELISA TCDD EQs were consistent with the dioxin TEQs as measured by GC/HRMS for 25 soil/sediment samples from seven different contaminated sites. The ELISA had an approximate method detection limit of 10 pg g(-1) with a precision of 2.6-29% based on the relative percentage difference (%RPD) for duplicate samples. Estimated sample throughput for the SPLE-ELISA was three times or more than that of the GC/HRMS method employing PLE with a multi-column cleanup. |
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Authors:
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Jane C Chuang; Jeanette M Van Emon; Mary E Schrock |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-09 |
Journal Detail:
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Title: Chemosphere Volume: 77 ISSN: 1879-1298 ISO Abbreviation: Chemosphere Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-28 Completed Date: 2010-02-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0320657 Medline TA: Chemosphere Country: England |
Other Details:
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Languages: eng Pagination: 1217-23 Citation Subset: IM |
Affiliation:
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Battelle, 505 King Avenue, Columbus, OH 43201-2693, USA. chuangj@battelle.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies
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immunology Dioxins / analysis*, chemistry Environmental Pollutants / analysis* Enzyme-Linked Immunosorbent Assay* Geologic Sediments / analysis* High-Throughput Screening Assays* Limit of Detection Soil / analysis* Soil Pollutants / analysis* Tetrachlorodibenzodioxin / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Dioxins; 0/Environmental Pollutants; 0/Soil; 0/Soil Pollutants; 1746-01-6/Tetrachlorodibenzodioxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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