Document Detail


High-throughput screening of dioxins in sediment and soil using selective pressurized liquid extraction with immunochemical detection.
MedLine Citation:
PMID:  19818991     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A high-throughput screening method using selective pressurized liquid extraction (SPLE) and enzyme-linked immunosorbent assay (ELISA) for monitoring dioxins in sediment and soil is described. SPLE conditions were developed by extracting sediment or soil together with alumina, 10% AgNO3 in silica, and sulfuric acid impregnated silica (acid silica) using dichloromethane (DCM) as the solvent at 100 degrees C and 2000 psi. Post-extraction cleanups were not required for ELISA. Two reference sediments (National Institute of Standards and Technology SRM 1944 and Wellington Laboratories WMS01) were analyzed by the SPLE-ELISA method. The ELISA utilized a polyclonal antibody and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as the calibrant. Recoveries of ELISA-derived TCDD equivalents (EQ) relative to the expected gas chromatography/high resolution mass spectrometry (GC/HRMS) derived dioxin toxic equivalent (TEQ) values were 116+/-11% for SRM 1944 and 102+/-13% for WMS01. ELISA TCDD EQs were consistent with the dioxin TEQs as measured by GC/HRMS for 25 soil/sediment samples from seven different contaminated sites. The ELISA had an approximate method detection limit of 10 pg g(-1) with a precision of 2.6-29% based on the relative percentage difference (%RPD) for duplicate samples. Estimated sample throughput for the SPLE-ELISA was three times or more than that of the GC/HRMS method employing PLE with a multi-column cleanup.
Authors:
Jane C Chuang; Jeanette M Van Emon; Mary E Schrock
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-09
Journal Detail:
Title:  Chemosphere     Volume:  77     ISSN:  1879-1298     ISO Abbreviation:  Chemosphere     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-28     Completed Date:  2010-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0320657     Medline TA:  Chemosphere     Country:  England    
Other Details:
Languages:  eng     Pagination:  1217-23     Citation Subset:  IM    
Affiliation:
Battelle, 505 King Avenue, Columbus, OH 43201-2693, USA. chuangj@battelle.org
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MeSH Terms
Descriptor/Qualifier:
Antibodies / immunology
Dioxins / analysis*,  chemistry
Environmental Pollutants / analysis*
Enzyme-Linked Immunosorbent Assay*
Geologic Sediments / analysis*
High-Throughput Screening Assays*
Limit of Detection
Soil / analysis*
Soil Pollutants / analysis*
Tetrachlorodibenzodioxin / chemistry
Chemical
Reg. No./Substance:
0/Antibodies; 0/Dioxins; 0/Environmental Pollutants; 0/Soil; 0/Soil Pollutants; 1746-01-6/Tetrachlorodibenzodioxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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