Document Detail


High throughput process development of chromatography steps: Advantages and limitations of different formats used.
MedLine Citation:
PMID:  22745056     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In the past, development of a chromatographic separation method has been accomplished by performing a series of experiments using either manual or automated chromatography systems. The screening of a vast experimental space became very expensive due to: all experiments had to be performed in a serial manner; the chromatography systems used were designed for relatively large columns; and therefore the experiments required large sample volumes. To address these issues, high throughput miniaturized methods employing different operating principles and/or formats have been introduced. In this work a technical review of the most common high-throughput formats used for development of chromatographic purification steps is presented. The formats considered include micro-columns, pre-filled pipette tips, and microtiter filter plates pre-filled with chromatography resins. Advantages and limitations of each format are discussed through the prism of chromatographic theory, engineering principles and known mass transfer mechanisms. A roadmap for applicability of the different formats for process development purposes and implementation of a Quality by Design initiative for designing/optimization of chromatography steps is also discussed.
Authors:
Karol M Lącki
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-29
Journal Detail:
Title:  Biotechnology journal     Volume:  -     ISSN:  1860-7314     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
GE Healthcare Bio-Sciences AB, SE-751 84 Uppsala, Sweden. karol.lacki@ge.com.
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