| High throughput process development of chromatography steps: Advantages and limitations of different formats used. | |
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MedLine Citation:
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PMID: 22745056 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In the past, development of a chromatographic separation method has been accomplished by performing a series of experiments using either manual or automated chromatography systems. The screening of a vast experimental space became very expensive due to: all experiments had to be performed in a serial manner; the chromatography systems used were designed for relatively large columns; and therefore the experiments required large sample volumes. To address these issues, high throughput miniaturized methods employing different operating principles and/or formats have been introduced. In this work a technical review of the most common high-throughput formats used for development of chromatographic purification steps is presented. The formats considered include micro-columns, pre-filled pipette tips, and microtiter filter plates pre-filled with chromatography resins. Advantages and limitations of each format are discussed through the prism of chromatographic theory, engineering principles and known mass transfer mechanisms. A roadmap for applicability of the different formats for process development purposes and implementation of a Quality by Design initiative for designing/optimization of chromatography steps is also discussed. |
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Authors:
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Karol M Lącki |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-29 |
Journal Detail:
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Title: Biotechnology journal Volume: - ISSN: 1860-7314 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101265833 Medline TA: Biotechnol J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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GE Healthcare Bio-Sciences AB, SE-751 84 Uppsala, Sweden. karol.lacki@ge.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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