Document Detail


High throughput, non-invasive and dynamic toxicity screening on adherent cells using respiratory measurements.
MedLine Citation:
PMID:  19427895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A dynamic respiration assay based on luminescence decay time detection of oxygen for high throughput toxicological assessment is presented. The method uses 24-well plates (OxoDishes) read with the help of a sensor dish reader placed in a humidified CO(2)-incubator. Adherent primary rat hepatocytes and the human hepatic cell line Hep G2 were exposed to known toxic compounds. Dissolved oxygen concentration, a measure of respiration, was measured with an oxygen sensor optode immobilized in the centre of each well. The cells were maintained in the dishes during the assay period and can afterwards be processed for further analyses. This dynamic, non-invasive measurement allowed calculation of 50% lethal concentrations (LC(50)) for any incubation time point giving concentration-time-dependent responses without further manipulation or removal of the cells from the incubator. Toxicokinetic profiles are compared with Sulforhodamine B assay, a common cytotoxicity assay. The novel assay is robust and flexible, very easy to carry out and provides continuous online respiration data reflecting dynamic toxicity responses. It can be adapted to any cell-based system and the calculated kinetics contributes to understanding of cell death mechanisms.
Authors:
Simone Beckers; Fozia Noor; Ursula M?ller-Vieira; Manuela Mayer; Alexander Strigun; Elmar Heinzle
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Publication Detail:
Type:  Journal Article     Date:  2009-05-08
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  24     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  686-94     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 Elsevier Ltd. All rights reserved.
Affiliation:
Campus A 1.5, Biochemical Engineering Department, Saarland University, Saarbr?cken, Germany. e.heinzle@mx.uni-saarland.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay / methods*
Cell Adhesion
Cell Line, Tumor
Cytotoxins / toxicity*
Drug Evaluation, Preclinical / methods
Hepatocytes / cytology,  drug effects*,  metabolism
Humans
Male
Oxygen / metabolism
Oxygen Consumption / drug effects,  physiology*
Pharmacokinetics
Rats
Rats, Wistar
Toxicity Tests / methods*
Chemical
Reg. No./Substance:
0/Cytotoxins; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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