| High throughput, non-invasive and dynamic toxicity screening on adherent cells using respiratory measurements. | |
| | |
MedLine Citation:
|
PMID: 19427895 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A dynamic respiration assay based on luminescence decay time detection of oxygen for high throughput toxicological assessment is presented. The method uses 24-well plates (OxoDishes) read with the help of a sensor dish reader placed in a humidified CO(2)-incubator. Adherent primary rat hepatocytes and the human hepatic cell line Hep G2 were exposed to known toxic compounds. Dissolved oxygen concentration, a measure of respiration, was measured with an oxygen sensor optode immobilized in the centre of each well. The cells were maintained in the dishes during the assay period and can afterwards be processed for further analyses. This dynamic, non-invasive measurement allowed calculation of 50% lethal concentrations (LC(50)) for any incubation time point giving concentration-time-dependent responses without further manipulation or removal of the cells from the incubator. Toxicokinetic profiles are compared with Sulforhodamine B assay, a common cytotoxicity assay. The novel assay is robust and flexible, very easy to carry out and provides continuous online respiration data reflecting dynamic toxicity responses. It can be adapted to any cell-based system and the calculated kinetics contributes to understanding of cell death mechanisms. |
| | |
Authors:
|
Simone Beckers; Fozia Noor; Ursula M?ller-Vieira; Manuela Mayer; Alexander Strigun; Elmar Heinzle |
Related Documents
:
|
1201215 - The concentration dependence of the oxygen affinity of haemoglobin s. 17914485 - Measuring the lifetime of singlet oxygen in a single cell: addressing the issue of cell... 20072895 - Intracellular atp delivery using highly fusogenic liposomes. 10370095 - Cell-cycle-dependent changes in membrane potential of l-929 cells caused by the effect ... 1722025 - Chromatin reorganization during senescence of proliferating cells. 8666595 - Changes in proteoglycan synthesis of chondrocytes in articular cartilage are associated... |
Publication Detail:
|
Type: Journal Article Date: 2009-05-08 |
Journal Detail:
|
Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 24 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-02-22 Completed Date: 2010-06-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
|
Languages: eng Pagination: 686-94 Citation Subset: IM |
Copyright Information:
|
Copyright (c) 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Campus A 1.5, Biochemical Engineering Department, Saarland University, Saarbr?cken, Germany. e.heinzle@mx.uni-saarland.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Assay / methods* Cell Adhesion Cell Line, Tumor Cytotoxins / toxicity* Drug Evaluation, Preclinical / methods Hepatocytes / cytology, drug effects*, metabolism Humans Male Oxygen / metabolism Oxygen Consumption / drug effects, physiology* Pharmacokinetics Rats Rats, Wistar Toxicity Tests / methods* |
| Chemical | |
Reg. No./Substance:
|
0/Cytotoxins; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of Pithecellobium clypearia Benth extract and its main components on inflammation and allerg...
Next Document: Assessment of methyl thiophanate-Cu (II) induced DNA damage in human lymphocytes.