Document Detail


High throughput microfluidic cell array for cytotoxicity screening.
MedLine Citation:
PMID:  19163275     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A microfluidic array with micro cell sieves was designed to fabricate multiple cells array for high-throughput cell cytotoxicity screening. Micro channels in both directions (column and row) were individually addressable by using a set of elastomeric monolithic array valves, enabling parallel loading of various cell lines in one direction and introducing of different toxins in the other direction. Several micro cell sieves were designed in each chamber. Cell number and distribution in chambers can be conveniently controlled by the adjustment of cell sieve number, distribution and size. Fluid transport property and cell loading in chamber were simulated and verified experimentally. The capability of the microfluidic array was demonstrated for seeding multiple mammalian cell lines in chambers and observing the cell viabilities after cells exposed to different toxins with a fluorescence microscope.
Authors:
Zhanhui Wang; Min-Cheol Kim; Todd Thorsen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference     Volume:  2008     ISSN:  1557-170X     ISO Abbreviation:  Conf Proc IEEE Eng Med Biol Soc     Publication Date:  2008  
Date Detail:
Created Date:  2009-02-16     Completed Date:  2009-04-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101243413     Medline TA:  Conf Proc IEEE Eng Med Biol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2752-5     Citation Subset:  IM    
Affiliation:
Mechanical Engineering Department, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. zh.wang@siat.ac.cn
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Cell Line
Computer Simulation
Cytotoxicity, Immunologic / drug effects*,  physiology
Equipment Design
Hela Cells
Humans
Mice
Mice, Inbred BALB C
Microfluidic Analytical Techniques / instrumentation,  methods*
Microfluidics / instrumentation,  methods*
Microscopy, Fluorescence / methods
NIH 3T3 Cells
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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