| High-throughput immunofluorescence microscopy using yeast spheroplast cell-based microarrays. | |
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MedLine Citation:
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PMID: 21104056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have described a protocol for performing high-throughput immunofluorescence microscopy on microarrays of yeast cells. This approach employs immunostaining of spheroplasted yeast cells printed as high-density cell microarrays, followed by imaging using automated microscopy. A yeast spheroplast microarray can contain more than 5,000 printed spots, each containing cells from a given yeast strain, and is thus suitable for genome-wide screens focusing on single cell phenotypes, such as systematic localization or co-localization studies or genetic assays for genes affecting probed targets. We demonstrate the use of yeast spheroplast microarrays to probe microtubule and spindle defects across a collection of yeast strains harboring tetracycline-down-regulatable alleles of essential genes. |
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Authors:
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Wei Niu; G Traver Hart; Edward M Marcotte |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 706 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2011 |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2011-03-22 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 83-95 Citation Subset: IM |
Affiliation:
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Department of Genetics, Yale University, New Haven, CT, USA. wei.niu08@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Down-Regulation Genes, Fungal High-Throughput Screening Assays / methods* Microscopy, Fluorescence Microtubules / metabolism Mitotic Spindle Apparatus / metabolism, pathology Spheroplasts / genetics* Tetracycline / pharmacology Tissue Array Analysis / methods* Yeasts / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM076536/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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60-54-8/Tetracycline |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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