Document Detail


High-throughput generation of tagged stable cell lines for proteomic analysis.
MedLine Citation:
PMID:  19405035     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present an optimized system for rapid generation of localization and affinity purification-tagged mammalian stable cell lines that facilitates complex purification and interacting protein identification. The improved components of this method, including the flexibility of inducible expression, circumvent issues associated with toxicity, clonal selection, sample yields and time to data acquisition. We have applied this method to the study of cell-cycle regulators and novel microtubule-associated proteins.
Authors:
Jorge Z Torres; Julie J Miller; Peter K Jackson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  9     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-08-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2888-91     Citation Subset:  IM    
Affiliation:
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. jorget@gene.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line*
Cloning, Molecular*
Mammals
Proteins / analysis*,  isolation & purification,  metabolism
Proteomics / methods*
Recombinant Fusion Proteins / isolation & purification,  metabolism
Grant Support
ID/Acronym/Agency:
R01 GM 54811/GM/NIGMS NIH HHS; R01 GM 60439/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Proteins; 0/Recombinant Fusion Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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