| High-throughput generation of tagged stable cell lines for proteomic analysis. | |
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MedLine Citation:
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PMID: 19405035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We present an optimized system for rapid generation of localization and affinity purification-tagged mammalian stable cell lines that facilitates complex purification and interacting protein identification. The improved components of this method, including the flexibility of inducible expression, circumvent issues associated with toxicity, clonal selection, sample yields and time to data acquisition. We have applied this method to the study of cell-cycle regulators and novel microtubule-associated proteins. |
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Authors:
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Jorge Z Torres; Julie J Miller; Peter K Jackson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proteomics Volume: 9 ISSN: 1615-9861 ISO Abbreviation: Proteomics Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-25 Completed Date: 2009-08-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 2888-91 Citation Subset: IM |
Affiliation:
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Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. jorget@gene.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line* Cloning, Molecular* Mammals Proteins / analysis*, isolation & purification, metabolism Proteomics / methods* Recombinant Fusion Proteins / isolation & purification, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM 54811/GM/NIGMS NIH HHS; R01 GM 60439/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 0/Recombinant Fusion Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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