Document Detail

High-throughput cellular screening of engineered ECM based on combinatorial polyelectrolyte multilayer films.
MedLine Citation:
PMID:  22632764     Owner:  NLM     Status:  MEDLINE    
The capacity to engineer the extracellular matrix is critical to better understand cell function and to design optimal cellular environments to support tissue engineering, transplantation and repair. Stacks of adsorbed polymers can be engineered as soft wet three dimensional matrices, with properties tailored to support cell survival and growth. Here, we have developed a combinatorial method to generate coatings that self assemble from solutions of polyelectrolytes in water, layer by layer, to produce a polyelectrolyte multilayer (PEM) coating that has enabled high-throughput screening for cellular biocompatibility. Two dimensional combinatorial PEMs were used to rapidly identify assembly conditions that promote optimal cell survival and viability. Conditions were first piloted using a cell line, human embryonic kidney 293 cells (HEK 293), and subsequently tested using primary cultures of embryonic rat spinal commissural neurons. Cell viability was correlated with surface energy (wettability), modulus (matrix stiffness), and surface charge of the coatings.Our findings indicate that the modulus is a crucial determinant of the capacity of a surface to inhibit or support cell survival.
Miloslav Sailer; Karen Lai Wing Sun; Ozzy Mermut; Timothy E Kennedy; Christopher J Barrett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-24
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-06-05     Completed Date:  2012-09-24     Revised Date:  2012-10-02    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  5841-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Department of Chemistry, Program in NeuroEngineering, McGill University, 801 Sherbrooke St. W., Montreal, Quebec H3A 2K6, Canada.
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MeSH Terms
Acrylic Resins / chemistry,  metabolism
Cell Differentiation
Cell Line
Cell Survival
Cells, Cultured
Coated Materials, Biocompatible / chemistry*,  metabolism*
Equipment Design
Extracellular Matrix / chemistry,  metabolism
HEK293 Cells
High-Throughput Screening Assays / instrumentation*,  methods
Materials Testing / instrumentation*,  methods
Neurons / cytology
Polyamines / chemistry,  metabolism
Grant Support
FRN79513//Canadian Institutes of Health Research
Reg. No./Substance:
0/Acrylic Resins; 0/Coated Materials, Biocompatible; 0/Polyamines; 9003-01-4/carbopol 940

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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