| High-throughput caveolar proteomic signature profile for maternal binge alcohol consumption. | |
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MedLine Citation:
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PMID: 20053519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Currently, no single marker is sensitive and specific enough to be considered a reliable biomarker for prenatal alcohol exposure. To identify a proteomic signature profile for maternal alcohol consumption, we carried out high-throughput proteomics on maternal endothelial caveolae exposed to moderate binge-like alcohol conditions. In these specialized lipid-ordered microdomains that contain a rich assembly of proteins, we demonstrate that moderate binge-like alcohol resulted in a distinctive maternal caveolar proteomic signature with important proteins being dramatically decreased/knocked out in the alcoholic profile. These proteins span from histones and basic structural proteins like α tubulin to proteins involved in trafficking, deubiquitination, cell signaling, and cell-cell adhesion. The profile also suggests an important role for the mother and the uteroplacental compartment in the pathogenesis of fetal alcohol spectrum disorders (FASD). These data demonstrate that the caveolar proteomic signature created by alcohol shows a promising direction for early detection of FASD. |
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Authors:
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Jayanth Ramadoss; Wu-xiang Liao; Dong-bao Chen; Ronald R Magness |
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Publication Detail:
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Type: Journal Article Date: 2010-01-06 |
Journal Detail:
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Title: Alcohol (Fayetteville, N.Y.) Volume: 44 ISSN: 1873-6823 ISO Abbreviation: Alcohol Publication Date: 2010 Nov-Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: 2011-03-11 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 8502311 Medline TA: Alcohol Country: United States |
Other Details:
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Languages: eng Pagination: 691-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Obstetrics and Gynecology, Perinatal Research Laboratories, University of Wisconsin, Atrium-B Meriter Hospital, 202 South Park Street Madison, WI 53715, USA. ramadoss@wisc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alcohol Drinking
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adverse effects* Animals Carrier Proteins / genetics Caveolae / chemistry* Caveolin 1 / analysis Disease Models, Animal Endothelium, Vascular / chemistry, ultrastructure* Ethanol / administration & dosage* Female Fetal Alcohol Syndrome / diagnosis Nitric Oxide Synthase Type III / analysis Pregnancy Proteomics* Sheep Signal Transduction / genetics Tubulin / analysis |
| Grant Support | |
ID/Acronym/Agency:
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K99 AA019446-02/AA/NIAAA NIH HHS; P01 HD038843-070002/HD/NICHD NIH HHS; R01 HL049210-11/HL/NHLBI NIH HHS; R01 HL070562-07/HL/NHLBI NIH HHS; R01 HL087144-03/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Caveolin 1; 0/Tubulin; 64-17-5/Ethanol; EC 1.14.13.39/Nitric Oxide Synthase Type III |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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