| High-throughput assay for small molecules that modulate zebrafish embryonic heart rate. | |
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MedLine Citation:
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PMID: 16408054 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate. |
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Authors:
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C Geoffrey Burns; David J Milan; Eric J Grande; Wolfgang Rottbauer; Calum A MacRae; Mark C Fishman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2005-09-18 |
Journal Detail:
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Title: Nature chemical biology Volume: 1 ISSN: 1552-4450 ISO Abbreviation: Nat. Chem. Biol. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2006-01-12 Completed Date: 2006-02-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 101231976 Medline TA: Nat Chem Biol Country: United States |
Other Details:
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Languages: eng Pagination: 263-4 Citation Subset: IM |
Affiliation:
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Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA. gburns@cvrc.mgh.harvard.edu |
| Data Bank Information | |
Bank Name/Acc. No.:
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PubChem-Substance/4266412; 4266413; 4266415; 4266416; 4266417 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Assay / methods* Cardiovascular Agents / toxicity* Dose-Response Relationship, Drug Drug Evaluation, Preclinical / methods Embryo, Nonmammalian / physiology Feasibility Studies Green Fluorescent Proteins / analysis* Heart / drug effects*, embryology, physiology* Heart Rate / drug effects*, physiology Myocardium / metabolism Time Factors Zebrafish |
| Grant Support | |
ID/Acronym/Agency:
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1K01AG023562-01/AG/NIA NIH HHS; 1R03NS050806-01/NS/NINDS NIH HHS; 5T32HL07208-26/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cardiovascular Agents; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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