Document Detail

High throughput analysis of red wine and grape phenolics-adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format.
MedLine Citation:
PMID:  17497877     Owner:  NLM     Status:  MEDLINE    
The methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers and Evans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of the MCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed. The Somers color assay was modified to allow the standardization of pH and ethanol concentrations of wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistencies between wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validation studies showed that all new formats were efficient, and results were reproducible and analogous to the original formats.
Meagan D Mercurio; Robert G Dambergs; Markus J Herderich; Paul A Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-12
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  55     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-06     Completed Date:  2007-09-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4651-7     Citation Subset:  IM    
The Australian Wine Research Institute, P.O. Box 197, Glen Osmond, SA, 5064, Australia.
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MeSH Terms
Catechin / analysis
Phenols / analysis*
Tannins / analysis*
Vitis / chemistry*
Wine / analysis*
Reg. No./Substance:
0/Phenols; 0/Tannins; 154-23-4/Catechin

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