Document Detail


High-throughput analysis of plasma fatty acid methyl esters employing robotic transesterification and fast gas chromatography.
MedLine Citation:
PMID:  18084789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fatty acid analysis is an important research tool, and indices derived from essential fatty acid contents serve as useful biomarkers related to cardiovascular and other chronic disease risk. Both clinical and basic studies of essential fatty acid composition are becoming ever larger in magnitude leading to delays while the rather laborious lipid analyses are performed. A robotic transesterification procedure has been developed for high-throughput analysis of plasma fatty acid methyl esters. In this approach, robots perform most steps including plasma and reagent transfer, transesterification reaction via heating at 80 degrees C in open tubes with multiple reagent additions, followed by two-phase extraction and transfer of lipid extracts to GC vials. The vials are then placed directly onto a GC autosampler carousel for robotic sample injection. An improved fast GC method is presented in which the peaks of interest are eluted within 6 min. This method is readily scalable to prepare and analyze 200 samples per day (1,000 samples per week) so that large clinical trials can be accommodated.
Authors:
M Athar Masood; Norman Salem
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Intramural     Date:  2007-12-15
Journal Detail:
Title:  Lipids     Volume:  43     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-07     Completed Date:  2008-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  171-80     Citation Subset:  IM    
Affiliation:
Laboratory of Membrane Biochemistry and Biophysics, National Institutes on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA. masoodaa@mail.nih.gov
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gas / methods*
Esterification
Esters / blood,  chemistry
Fatty Acids / blood*
Reproducibility of Results
Robotics*
Chemical
Reg. No./Substance:
0/Esters; 0/Fatty Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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