| High-throughput analysis of mouse embryos by magnetic resonance imaging. | |
| | |
MedLine Citation:
|
PMID: 22194264 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Genetic studies in the mouse are crucial for uncovering new genes and signaling pathways associated with development. The identification of murine models with developmental malformations in high-throughput mutagenesis screens is made difficult because, after mid-embryogenesis, the embryo is opaque. Traditional phenotyping methods such as histological sectioning are labor intensive and destructive. We have developed and optimized a novel method for high-throughput multiembryo magnetic resonance imaging (MRI). Here we present our method for processing 32 mouse embryos for analysis by MRI. We describe the MR system, imaging software, and the reconstruction of two-dimensional (2D) and three-dimensional (3D) images. We also discuss the applications of this technique, highlight its advantages, and point out some disadvantages. Using this approach, we can identify developmental malformations in mutant embryos at high spatial resolution (voxel size 25.4 × 25.4 × 24.4 µm). This technique can be easily used for mouse mutagenesis screens and thus provides an important tool for identifying new mouse models for human diseases. |
| | |
Authors:
|
Simon D Bamforth; Jürgen E Schneider; Shoumo Bhattacharya |
Related Documents
:
|
16958414 - Small bowel perforation resulting from blunt abdominal trauma: interval change of radio... 10029364 - Imaging studies in dyspepsia. 11598254 - Ct of the duodenum: an overlooked segment gets its due. 9238794 - Scintigraphic evaluation of four dogs with protein-losing enteropathy using 111indium-l... 18639994 - Assessment of congenital heart disease (chd): is there a role for fetal magnetic resona... 22480944 - Theoretical and experimental characterisation of magnetic microbubbles. |
Publication Detail:
|
Type: Journal Article Date: 2012-01-01 |
Journal Detail:
|
Title: Cold Spring Harbor protocols Volume: 2012 ISSN: 1559-6095 ISO Abbreviation: Cold Spring Harb Protoc Publication Date: 2012 |
Date Detail:
|
Created Date: 2011-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101524530 Medline TA: Cold Spring Harb Protoc Country: United States |
Other Details:
|
Languages: eng Pagination: - Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: BAPTI and BAPTISM Birthdating of Neurons in Zebrafish.
Next Document: Production, Harvest, and Concentration of Feline Immunodeficiency Virus-Based Lentiviral Vector from...