| High-throughput SNP detection based on PCR amplification on magnetic nanoparticles using dual-color hybridization. | |
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MedLine Citation:
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PMID: 19768607 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A microarray-based method for detecting single nucleotide polymorphisms (SNPs) using solid-phase polymerase chain reaction (PCR) on magnetic nanoparticles (MNPs) was developed. In this method one primer with a biotin label is captured by streptavidin-coated MNPs (SA-MNPs), and the PCR products are directly amplified on the surface of SA-MNPs in a 96-well plate. The samples are further probed by hybridization with a pair of dual-color probes to determine SNP. The genotype of each sample can be simultaneously identified by scanning the microarray printed with the denatured fluorescent probes. All the reactions can be performed in the same reaction volume without the necessity of purification of intermediates. This approach represents a novel, simple, and labor-saving method for SNP genotyping and can be applied in automation system(s) to achieve high-throughput SNP detection. |
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Authors:
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Nongyue He; Song Li; Hongna Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 578 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-09-21 Completed Date: 2009-12-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 393-402 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Color Electrophoresis, Agar Gel Genotype High-Throughput Screening Assays / methods* Humans Magnetics / methods* Nanoparticles / chemistry* Nucleic Acid Hybridization / methods* Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction / methods* Polymorphism, Single Nucleotide / genetics* Streptavidin / metabolism |
| Chemical | |
Reg. No./Substance:
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9013-20-1/Streptavidin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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