| High temporal resolution coupling of low-flow push-pull perfusion to capillary electrophoresis for ascorbate analysis at the rat vitreoretinal interface. | |
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MedLine Citation:
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PMID: 19173069 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A system is presented demonstrating the high-temporal resolution coupling of low-flow push-pull perfusion sampling (LFPS) to capillary electrophoresis for the absorbance measurement of ascorbate at the rat vitreoretinal interface. This system holds all separation components at a low pressure as the means for withdrawing sample during LFPS. The system uses a flow-gated interface to directly couple the withdrawal capillary from the LFPS probe to a separation capillary and eliminates the need for any offline sample handling. The temporal resolution of the system was limited by injection time and is less than 16 s. This high temporal resolution was applied to the monitoring of in vivo ascorbate levels at the rat vitreoretinal interface. Baseline concentrations of ascorbate were found to be 86 microM +/- 18 microM at the vitreoretinal interface. Baseline concentrations matched well with those obtained for the postmortem bulk vitreous analysis. Upon stimulation with 145 mM K(+), a maximum increase in baseline values between 32-107% for n = 3 was observed. This system demonstrates the first in vivo temporal study of ascorbate at the rat vitreoretinal interface. |
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Authors:
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Eric E Patterson; Jeanita S Pritchett; Scott A Shippy |
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Publication Detail:
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Type: Journal Article Date: 2008-11-27 |
Journal Detail:
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Title: The Analyst Volume: 134 ISSN: 1364-5528 ISO Abbreviation: Analyst Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-28 Completed Date: 2009-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: England |
Other Details:
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Languages: eng Pagination: 401-6 Citation Subset: IM |
Affiliation:
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Department of Chemistry (M/C 111), University of Illinois at Chicago, 845 W. Taylor St. Room 4500, Chicago, Illinois 60607-7061, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascorbic Acid / analysis* Electrophoresis, Capillary / methods Flow Injection Analysis / methods Male Perfusion Rats Rats, Long-Evans Retina / chemistry* Vitreous Body / chemistry* |
| Chemical | |
Reg. No./Substance:
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50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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