| High survival rate of unfertilized mouse oocytes after vitrification. | |
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MedLine Citation:
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PMID: 2600904 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Unfertilized mouse oocytes were cooled rapidly by directly plunging them into liquid nitrogen, immediately after exposure to a highly concentrated solution (modified VS1: 2.53 M-dimethyl sulphoxide, 2.36 M-acetamide, 1.19 M-propylene glycol, 5.4% (w/v) polyethylene glycol (Mr 8000) in PB1), and later warmed in a 37 degree C waterbath. After warming, 305 out of 348 oocytes (87.6%) were morphologically normal. After fertilization in vitro of cryopreserved oocytes, the proportions of pronuclear oocytes and 2-cell embryos 5 and 24 h after insemination were 81.6% (124/152) and 78.4% (120/153), respectively. All 2-cell embryos obtained from cryopreserved oocytes were transferred to the oviducts of pseudopregnant recipients and 45.8% (55/120) developed to normal young. |
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Authors:
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N Nakagata |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 87 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1989 Nov |
Date Detail:
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Created Date: 1990-02-08 Completed Date: 1990-02-08 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 479-83 Citation Subset: IM |
Affiliation:
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Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Cryopreservation / methods* Embryo Transfer Female Fertilization in Vitro Hybridization, Genetic Mice Mice, Inbred C3H Mice, Inbred C57BL Oocytes / cytology, physiology* |
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