Document Detail

High survival rate of unfertilized mouse oocytes after vitrification.
MedLine Citation:
PMID:  2600904     Owner:  NLM     Status:  MEDLINE    
Unfertilized mouse oocytes were cooled rapidly by directly plunging them into liquid nitrogen, immediately after exposure to a highly concentrated solution (modified VS1: 2.53 M-dimethyl sulphoxide, 2.36 M-acetamide, 1.19 M-propylene glycol, 5.4% (w/v) polyethylene glycol (Mr 8000) in PB1), and later warmed in a 37 degree C waterbath. After warming, 305 out of 348 oocytes (87.6%) were morphologically normal. After fertilization in vitro of cryopreserved oocytes, the proportions of pronuclear oocytes and 2-cell embryos 5 and 24 h after insemination were 81.6% (124/152) and 78.4% (120/153), respectively. All 2-cell embryos obtained from cryopreserved oocytes were transferred to the oviducts of pseudopregnant recipients and 45.8% (55/120) developed to normal young.
N Nakagata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  87     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-02-08     Completed Date:  1990-02-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  479-83     Citation Subset:  IM    
Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo, Japan.
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MeSH Terms
Cell Survival
Cryopreservation / methods*
Embryo Transfer
Fertilization in Vitro
Hybridization, Genetic
Mice, Inbred C3H
Mice, Inbred C57BL
Oocytes / cytology,  physiology*

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