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HIGH SURVIVAL OF FROZEN CELLS IRRADIATED WITH GAMMA RADIATION.
MedLine Citation:
PMID:  21186220     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cell storage in liquid nitrogen (LN) offers the most secure method of cell preservation even if cryopreserved cells are exposed to natural background of ionising radiation (IR). A lot of experiments have demonstrated that IR can induce damages in living cells, but only a little information regarding the response of cryopreserved cells is available. To investigate the effect of IR on frozen and unfrozen cells, peripheral blood mononuclear cells were directly irradiated at room temperature, then immediately frozen, or frozen and then irradiated in LN with different doses of gamma rays. After thawing, cells were incubated and death fraction was evaluated at different time points. Interestingly, the percentages of dead cells induced by IR gradually increased with both dose radiation and incubation time and were significantly lower for cells irradiated at -196°C than those irradiated at room temperature.
Authors:
Giulia Cugia; Filippo Centis; Genny Del Zotto; Alessandra Lucarini; Elisabetta Argazzi; Gianpaolo Zini; Massimo Valentini; Monica Bono; Francesco Picardi; Stefania Stramigioli; Werther Cesarini; Loris Zamai
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-24
Journal Detail:
Title:  Radiation protection dosimetry     Volume:  -     ISSN:  1742-3406     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109958     Medline TA:  Radiat Prot Dosimetry     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Human, Environmental and Natural Sciences, University of Urbino 'Carlo Bo', via Ca Le Suore, 2/4, 61029 Urbino (PU), Italy.
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