Document Detail

High-speed, whole-column fluorescence imaging detection for isoelectric focusing on a microchip using an organic light emitting diode as light source.
MedLine Citation:
PMID:  16906731     Owner:  NLM     Status:  MEDLINE    
An integrated and simplified microfluidic device using a 250 microm x 1-4 cm of organic light emitting diode (OLED) array as a two-dimensional light source for single-channel and multichannel whole-column imaging detection was developed. This fluorescence detection system was used for isoelectric focusing (IEF) of R-phycoerythrin in a microchip. The IEF conditions were optimized, and the total analysis time was extremely reduced to 30 s for 2-cm-long microchannels at 700 V/cm of electric field strength without the presence of electroosmotic flow. The compression of pH gradient caused by electrolytes drawing into the microchannels was efficiently restrained when 1% hydroxylpropylmethyl cellulose in 2% ampholyte was used as the carrier for IEF. Under optimized IEF conditions, the detection limit of this system was approximately 0.6 microg/mL or 45 pg at 75 nL/column injection of R-phycoerythrin. This OLED-induced fluorescence detection system for WCID provides a high-speed IEF technique with quantitative ability and the potential for high integration and throughput microchip systems.
Bo Yao; Huihua Yang; Qionglin Liang; Guoan Luo; Liduo Wang; Kangning Ren; Yudi Gao; Yiming Wang; Yong Qiu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  78     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-08-15     Completed Date:  2007-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5845-50     Citation Subset:  IM    
Department of Chemistry, Tsinghua University, Beijing 100084, China.
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MeSH Terms
Isoelectric Focusing / methods*
Microfluidic Analytical Techniques / methods*
Phycoerythrin / analysis
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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