| High spatial resolution measurements of organ blood flow in small laboratory animals. | |
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MedLine Citation:
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PMID: 11045936 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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With the use of a newly developed Imaging Cryomicrotome to determine the spatial location of fluorescent microspheres in organs, we validate and report our processing algorithms for measuring regional blood flow in small laboratory animals. Microspheres (15-microm diameter) of four different fluorescent colors and one radioactive label were simultaneously injected into the left ventricle of a pig. The heart and kidneys were dissected, and the numbers of fluorescent and radioactive microspheres were determined in 10 randomly selected pieces. All microsphere counts fell well within the 95% expected confidence limits as determined from the radioactive counts. Fluorescent microspheres (15-microm diameter) of four different colors were also injected into the tail vein of a rat and the left ventricle of a rabbit. After correction for Poisson noise, correlation coefficients between the colors were 0.99 +/- 0.02 (means +/- SD) for the rabbit heart and 0.99 +/- 0.02 for the rat lung. Mathematical dissection algorithms, statistics to analyze the spatial data, and methods to visualize blood flow distributions in small animal organs are presented. |
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Authors:
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S L Bernard; J R Ewen; C H Barlow; J J Kelly; S McKinney; D A Frazer; R W Glenny |
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Publication Detail:
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Type: Journal Article; Validation Studies |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 279 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-11-17 Completed Date: 2000-12-28 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: H2043-52 Citation Subset: IM |
Affiliation:
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Division of Pulmonary and Critical Care Medicine, University of Washington, Seattle 98195, USA. sbernard@u.washington.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Flow Velocity* Fluorescent Dyes / pharmacokinetics Kidney / blood supply*, cytology Lung / blood supply*, cytology Microspheres Models, Cardiovascular Myocardium / metabolism* Poisson Distribution Rabbits Rats Regional Blood Flow / physiology* Swine Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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