Document Detail


High sensitivity of fetal DNA in plasma compared to serum and nucleated cells using unnested PCR in maternal blood.
MedLine Citation:
PMID:  10720875     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA analysis of blood is conventionally performed on cells - plasma and serum are discarded. Free DNA has been demonstrated in serum in cancer and autoimmune disorders and in pregnancy. We investigated possible noninvasive prenatal diagnosis using fetal DNA from maternal plasma and serum in pregnancy. Fetal gender was determined by PCR on DNA from maternal venous blood, serum and plasma of 65 women by boiling with or without phenol/chloroform extraction. When sensitivities were compared for plasma, additional phenol/chloroform extraction proved more sensitive than boiling alone (89 vs. 50%), the observed difference was 50% (CI 19 to 81%). Extracted plasma amplified better than extracted serum (89 vs. 46%), the observed difference being 44% (CI 22 to 66%). In contrast, fetal gender could not reliably be determined from DNA extracted from maternal nucleated blood cells. The size of plasma and serum DNA at 15-17 weeks of gestation was >1,500 bp. This work confirms the presence of fetal DNA in maternal plasma and serum which may be applicable to noninvasive prenatal diagnosis of paternally derived DNA sequences. We conclude that optimal sensitivity requires two methods of DNA extraction and that the use of plasma is preferred to that of serum.
Authors:
V Houfflin-Debarge; H O'Donnell; T Overton; P R Bennett; N M Fisk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Fetal diagnosis and therapy     Volume:  15     ISSN:  1015-3837     ISO Abbreviation:  Fetal. Diagn. Ther.     Publication Date:    2000 Mar-Apr
Date Detail:
Created Date:  2000-05-05     Completed Date:  2000-05-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9107463     Medline TA:  Fetal Diagn Ther     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  102-7     Citation Subset:  IM    
Copyright Information:
Copyright 2000 S. Karger AG, Basel.
Affiliation:
Institute of Obstetrics and Gynaecology, Department of Materno-Fetal Medicine, Imperial College School of Medicine, Queen Charlotte's and Chelsea Hospital, London, UK.
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MeSH Terms
Descriptor/Qualifier:
Chloroform
DNA / blood*
Female
Fetus*
Gestational Age
Hot Temperature
Humans
Phenol
Polymerase Chain Reaction
Pregnancy
Prenatal Diagnosis*
Sensitivity and Specificity
Sex Determination (Analysis)
Chemical
Reg. No./Substance:
108-95-2/Phenol; 67-66-3/Chloroform; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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