| High sensitivity biosensor measurement based on synchronous detection. | |
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MedLine Citation:
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PMID: 21196122 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The principle of synchronous detection (SD) has been applied to biosensor measurement. SD principle achieves significant increases in the signal to noise ratio, limit of detection and overall measurement robustness. Application of SD in biosensor measurement improves the analysis of the response and avoids the influence of interference/noise produced by stirring, electromagnetic effects and influence of parasitic currents. SD also enables the decomposition of signal to stimulation response and phenomena with long time of response. Second-order phenomena are identifiable in the signal. Linear statistical model was used to develop software for identification of the stimulation signal in the output current. SD was applied to the response signal of a Photosystem II complex (PSII) biosensor. PSII response to light stimulation follows first order kinetics. The inhibition kinetics of PSII has been studied. Kinetic constants of herbicide binding to PSII depend linearly on herbicide concentration and enable measurement of its concentration at low concentrations (linear range for diuron is 10(-6) to 10(-4)mM). |
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Authors:
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J Krejci; V Ondruch; J Maly; M Stofik; D Krejcova; H Vranova |
Publication Detail:
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Type: Journal Article Date: 2010-12-15 |
Journal Detail:
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Title: Journal of photochemistry and photobiology. B, Biology Volume: 102 ISSN: 1873-2682 ISO Abbreviation: J. Photochem. Photobiol. B, Biol. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8804966 Medline TA: J Photochem Photobiol B Country: Switzerland |
Other Details:
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Languages: eng Pagination: 192-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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BVT Technologies a.s. Hudcova 78, CZ-612 00 Brno, Czech Republic. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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