Document Detail


High-resolution optical mapping of intramural virtual electrodes in porcine left ventricular wall.
MedLine Citation:
PMID:  15537498     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: It is believed that shock-induced intramural virtual electrodes (IVE) play a critical role in defibrillation. IVE were recently demonstrated in the porcine left ventricle (LV), but their origin remains unknown. Macroscopic optical mapping showed that strong shocks induce IVE of only one polarity, which contradicts theoretical predictions. It is hypothesized that IVE have a microscopic origin and that microscopic positive and negative IVE are spatially averaged during macroscopic optical mapping. This hypothesis was examined by mapping V(m) responses at the transmural LV surface with increased optical resolution. METHODS: Rectangular shocks (strength=2-48 V/cm; duration=10 ms) were applied across isolated coronary-perfused porcine LV preparations (n=7) during the action potential plateau and diastole. Shock-induced V(m) responses were measured at low resolution (LR; 1.2 mm/diode) and high resolution (HR; 0.11 mm/diode). RESULTS: During plateau shocks with strength > or =20 V/cm, LR recordings demonstrated only negative DeltaV(m) extending to the cathodal preparation edge. In contrast, HR recordings from this area as well as from intramural locations revealed both positive and negative DeltaV(m) at all shock strengths. During diastolic shocks, only positive polarizations were observed at LR, but both positive and negative polarizations were detected at HR. In areas of negative polarization, large activation delays were found at HR, whereas LR recordings at these locations demonstrated fast activation. CONCLUSIONS: High- and low-resolution optical mapping produced radically different patterns of shock-induced polarization and activation. The occurrence of positive and negative polarizations during plateau and diastolic shocks at high but not low resolution provides evidence for microscopic nature of IVE in LV wall.
Authors:
Oleg F Sharifov; Raymond E Ideker; Vladimir G Fast
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cardiovascular research     Volume:  64     ISSN:  0008-6363     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-11     Completed Date:  2005-02-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  448-56     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, University of Alabama at Birmingham, 1670 University Boulevard, VH B126, Birmingham, AL 35294, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Coloring Agents
Electric Countershock*
Electrophysiology
Female
Male
Microscopy, Fluorescence
Models, Animal
Swine
Ventricular Dysfunction, Left / physiopathology*
Ventricular Fibrillation / physiopathology,  therapy*
Grant Support
ID/Acronym/Agency:
HL 67748/HL/NHLBI NIH HHS; HL 67961/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Coloring Agents
Comments/Corrections
Comment In:
Cardiovasc Res. 2004 Dec 1;64(3):384-6   [PMID:  15537490 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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