Document Detail

High-resolution measurement of breaks in prematurely condensed chromosomes by differential staining.
MedLine Citation:
PMID:  6653204     Owner:  NLM     Status:  MEDLINE    
A relatively simple method has been developed to improve the resolution for measuring breaks produced in interphase chromosomes by X rays or other agents following the induction of premature chromosome condensation (PCC). Mitotic HeLa cells, which induce PCC when fused with interphase cells, were obtained from cultures grown for several generations in 5-bromodeoxyuridine (BrdU). These were fused to cells from low-passage confluent cultures of normal human fibroblasts and subsequently stained by a modified fluorescence-plus-Giemsa (FPG) technique. Following this protocol the prematurely condensed chromosomes stain intensely, whereas the mitotic chromosomes of the inducer cell(s), which are intermingled with them, stain very lightly. With this technique the interphase chromosomes and their fragments can be identified unequivocally, making scoring much easier and more accurate. The frequency of breaks produced in G1 phase AG1522 human fibroblasts immediately following X-ray doses of 58 and 117 rad was 3.68 and 7.38 per cell, respectively. Use of this technique should allow the detection of damage from ionizing radiation at doses lower than 10 rad.
M N Cornforth; J S Bedford
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chromosoma     Volume:  88     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1983  
Date Detail:
Created Date:  1984-02-24     Completed Date:  1984-02-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  315-8     Citation Subset:  IM    
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MeSH Terms
Azure Stains / diagnostic use
Cell Cycle
Cell Line
Chromosome Aberrations*
Chromosomes / radiation effects,  ultrastructure*
Hybrid Cells / ultrastructure
Grant Support
Reg. No./Substance:
0/Azure Stains

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